Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite DNA

Akihisa Osakabe, Fumiya Adachi, Yasuhiro Arimura, Kazumitsu Maehara, Yasuyuki Ohkawa, Hitoshi Kurumizaka

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

DNA methylation occurs on CpG sites and is important to form pericentric heterochromatin domains. The satellite 2 sequence, containing seven CpG sites, is located in the pericentric region of human chromosome 1 and is highly methylated in normal cells. In contrast, the satellite 2 region is reportedly hypomethylated in cancer cells, suggesting that the methylation status may affect the chromatin structure around the pericentric regions in tumours. In this study, we mapped the nucleosome positioning on the satellite 2 sequence in vitro and found that DNA methylation modestly affects the distribution of the nucleosome positioning. The micrococcal nuclease assay revealed that the DNA end flexibility of the nucleosomes changes, depending on the DNA methylation status. However, the structures and thermal stabilities of the nucleosomes are unaffected by DNA methyl-ation. These findings provide new information to understand how DNA methylation functions in regulating pericentric heterochromatin formation and maintenance in normal and malignant cells.

Original languageEnglish
Article number150128
JournalOpen Biology
Volume5
Issue number10
DOIs
Publication statusPublished - 2015 Oct 7

Keywords

  • Chromatin
  • DNA methylation
  • Nucleosome
  • Pericentric heterochromatin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology

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