Influence of environmental light-dark cycle and enucleation on activity of suprachiasmatic neurons in slice preparations

Shigenobu Shibata; Yuh Liou Shyh; Showa Ueki; Yutaka Oomura

doi:10.1016/0006-8993(84)91286-1

Influence of environmental light-dark cycle and enucleation on activity of suprachiasmatic neurons in slice preparations

Shigenobu Shibata^*, Yuh Liou Shyh, Showa Ueki, Yutaka Oomura

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

93 Citations (Scopus)

Abstract

The influence of environmental light-dark cycle (LD) and bilateral enucleation on single neuronal activity in the suprachiasmatic nucleus (SCN) was examined using a hypothalamic slice preparation. Firstly, we reconfirmed previous results that the discharge rate in slices taken from animals kept on normal LD was higher during the light than during the dark period. Secondly, the day time discharge rate in the ventrolateral part of the SCN was decreased by bilateral enucleation and DD housing, while in the dorsomedial part it was unaffected. Thirdly, LL housing suppressed the discharge rates in both parts during the day and night periods. The present results suggest that the dorsomedial part of the SCN is more important in regulation of the circadian rhythm of SCN neuronal activity than the ventrolateral.

Original language	English
Pages (from-to)	75-81
Number of pages	7
Journal	Brain Research
Volume	302
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(84)91286-1
Publication status	Published - 1984 Jun 4
Externally published	Yes

Keywords

circadian rhythm
neuronal activity
rat brain slices
suprachiasmatic nucleus

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(84)91286-1

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@article{f1bdd287164e4241839ef11a41da8895,

title = "Influence of environmental light-dark cycle and enucleation on activity of suprachiasmatic neurons in slice preparations",

abstract = "The influence of environmental light-dark cycle (LD) and bilateral enucleation on single neuronal activity in the suprachiasmatic nucleus (SCN) was examined using a hypothalamic slice preparation. Firstly, we reconfirmed previous results that the discharge rate in slices taken from animals kept on normal LD was higher during the light than during the dark period. Secondly, the day time discharge rate in the ventrolateral part of the SCN was decreased by bilateral enucleation and DD housing, while in the dorsomedial part it was unaffected. Thirdly, LL housing suppressed the discharge rates in both parts during the day and night periods. The present results suggest that the dorsomedial part of the SCN is more important in regulation of the circadian rhythm of SCN neuronal activity than the ventrolateral.",

keywords = "circadian rhythm, neuronal activity, rat brain slices, suprachiasmatic nucleus",

author = "Shigenobu Shibata and Shyh, {Yuh Liou} and Showa Ueki and Yutaka Oomura",

note = "Funding Information: We thank Prof. A. Simpson, Showa University, for help in preparation of this manuscript. This work was supported by the Grant-in-Aid 587035, 00587035 (Y.O.) from the Ministry of Education, Science and Culture of Japan.",

year = "1984",

month = jun,

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doi = "10.1016/0006-8993(84)91286-1",

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T1 - Influence of environmental light-dark cycle and enucleation on activity of suprachiasmatic neurons in slice preparations

AU - Shibata, Shigenobu

AU - Shyh, Yuh Liou

AU - Ueki, Showa

AU - Oomura, Yutaka

N1 - Funding Information: We thank Prof. A. Simpson, Showa University, for help in preparation of this manuscript. This work was supported by the Grant-in-Aid 587035, 00587035 (Y.O.) from the Ministry of Education, Science and Culture of Japan.

PY - 1984/6/4

Y1 - 1984/6/4

N2 - The influence of environmental light-dark cycle (LD) and bilateral enucleation on single neuronal activity in the suprachiasmatic nucleus (SCN) was examined using a hypothalamic slice preparation. Firstly, we reconfirmed previous results that the discharge rate in slices taken from animals kept on normal LD was higher during the light than during the dark period. Secondly, the day time discharge rate in the ventrolateral part of the SCN was decreased by bilateral enucleation and DD housing, while in the dorsomedial part it was unaffected. Thirdly, LL housing suppressed the discharge rates in both parts during the day and night periods. The present results suggest that the dorsomedial part of the SCN is more important in regulation of the circadian rhythm of SCN neuronal activity than the ventrolateral.

AB - The influence of environmental light-dark cycle (LD) and bilateral enucleation on single neuronal activity in the suprachiasmatic nucleus (SCN) was examined using a hypothalamic slice preparation. Firstly, we reconfirmed previous results that the discharge rate in slices taken from animals kept on normal LD was higher during the light than during the dark period. Secondly, the day time discharge rate in the ventrolateral part of the SCN was decreased by bilateral enucleation and DD housing, while in the dorsomedial part it was unaffected. Thirdly, LL housing suppressed the discharge rates in both parts during the day and night periods. The present results suggest that the dorsomedial part of the SCN is more important in regulation of the circadian rhythm of SCN neuronal activity than the ventrolateral.

KW - circadian rhythm

KW - neuronal activity

KW - rat brain slices

KW - suprachiasmatic nucleus

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JO - Brain Research

JF - Brain Research

IS - 1

ER -

Influence of environmental light-dark cycle and enucleation on activity of suprachiasmatic neurons in slice preparations

Abstract

Keywords

ASJC Scopus subject areas

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