Influence of growth phase on bacterial cell electrokinetic characteristics examined by soft particle electrophoresis theory

Hiroshi Hayashi, Hiroshi Seiki, Satoshi Tsuneda, Akira Hirata, Hiroshi Sasaki

    Research output: Contribution to journalArticle

    35 Citations (Scopus)

    Abstract

    The influence of incubation time on the electrokinetic properties of Escherichia coli, Pseudomonas putida, Alcaligenes faecalis, and Alcaligenes sp., was examined by electrophoretic mobility measurements and the results were discussed based on Ohshima's soft particle theory. The electrophoretic mobility of E. coli plotted against incubation time revealed that the mobility gradually increased from the outset of incubation to 7 h, which corresponded to the exponential growth and early stationary phase, and then decreased. For all strains, electrophoretic mobility leveled off to nonzero values in accordance with the increase in ionic concentration, which was a characteristic feature of soft particles. Soft particle analysis was carried out at the outset of incubation and the time when mobility reached a maximum in order to obtain the spatial charge density (ZN) and cell surface softness (1/λ). ZN at the maximum electrophoretic mobility was more negative than that at the outset of incubation for E. coli, P. putida, and A. faecalis, while the ZN of Alcaligenes sp. almost remained unchanged. 1/λ decreased for E. coli, but increased for Alcaligenes sp. and A. faecalis. These findings indicated that the cell growth phase affects both ZN and 1/λ, although the dependencies of these parameters are unique to each bacterial strain.

    Original languageEnglish
    Pages (from-to)565-568
    Number of pages4
    JournalJournal of Colloid and Interface Science
    Volume264
    Issue number2
    DOIs
    Publication statusPublished - 2003 Aug 15

    Fingerprint

    Electrophoretic mobility
    electrokinetics
    electrophoresis
    Electrophoresis
    Escherichia coli
    cells
    Cell growth
    Charge density
    particle theory
    softness
    pseudomonas
    Escherichia

    Keywords

    • Bacterial cell
    • Cell growth phase
    • Cell surface potential
    • Electrophoretic mobility
    • Soft particle theory
    • Zeta potential

    ASJC Scopus subject areas

    • Colloid and Surface Chemistry
    • Physical and Theoretical Chemistry
    • Surfaces and Interfaces

    Cite this

    Influence of growth phase on bacterial cell electrokinetic characteristics examined by soft particle electrophoresis theory. / Hayashi, Hiroshi; Seiki, Hiroshi; Tsuneda, Satoshi; Hirata, Akira; Sasaki, Hiroshi.

    In: Journal of Colloid and Interface Science, Vol. 264, No. 2, 15.08.2003, p. 565-568.

    Research output: Contribution to journalArticle

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