Influence of hemoglobin vesicles, cellular-type artificial oxygen carriers, on human umbilical cord blood hematopoietic progenitor cells in vitro

Miki Yamaguchi, Mitsuhiro Fujihara, Shinobu Wakamoto, Hiromi Sakai, Shinji Takeoka, Eishun Tsuchida, Hiroshi Azuma, Hisami Ikeda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Hemoglobin vesicles (HbVs), liposomal oxygen carriers containing human hemoglobin, are candidates for development as clinically useful blood substitutes. Although HbVs are shown to distribute transiently into the bone marrow in animal models, the influence of HbVs on human hematopoietic stem/progenitor cells has not yet been studied. Therefore, we investigated the influence of HbVs at a concentration of up to 3 vol/vol % on the clonogenic activity (in semisolid culture) and proliferative activity (in liquid culture) of human hematopoietic progenitor cells derived from umbilical cord blood (CB) in vitro. Continuous exposure of CB mononuclear cells to HbVs tended to decrease the number and size of mature-committed colonies and most notably reduced the number of colonies of high-proliferative potential colony-forming cells (HPP-CFC). In contrast, exposure to HbVs for 20 h or 3 days, which is more relevant to the clinical setting, had no effect on the number of mature-committed colonies and only modestly decreased the number of HPP-CFC. Continuous exposure (10 days) to HbVs significantly suppressed the cellular proliferation and differentiation of both the erythroid and myeloid lineages in liquid culture. Again, short exposure (20 h or 3 days) did not affect these parameters. Thus, our results show that HbVs, under conditions relevant to the clinical setting, have no adverse effect on human CB hematopoietic progenitor activity in vitro.

Original languageEnglish
Pages (from-to)34-42
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume88
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1

Keywords

  • Biocompatibility
  • Colony assay
  • Hematopoietic progenitor cells
  • Hemoglobin-vesicles
  • Liposome-encapsulated hemoglobin

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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