Influence of nanometric holes on the sensitivity of a waveguide-mode sensor

Label-free nanosensor for the analysis of RNA aptamer-ligand interactions

Subash C B Gopinath, Koichi Awazu, Makoto Fujimaki, Katsuaki Sugimoto, Yoshimichi Ohki, Tetsuro Komatsubara, Junji Tominaga, Kailash C. Gupta, Penmetcha K R Kumar

    Research output: Contribution to journalArticle

    42 Citations (Scopus)

    Abstract

    Evanescent-field-coupled (EFC) waveguide-mode sensors can be used to detect nucleic acids or proteins from the changes in the local index of refraction upon adsorption of the target molecule on a waveguide surface. We recently described an EFC waveguide-mode sensor in which nanometric holes on a waveguide film resulted in an improved sensitivity in the analysis of the interactions of biomolecules. In the present study, we have shown that sensitivity depends upon the diameter of the holes, where increase in diameter of holes increases spectral shift resulting in an improved sensitivity. Using this improved EFC waveguide-mode sensor, we could detect interactions between RNA and a small ligand, cyanocobalamin (vitamin B12), and between RNA and a protein (human coagulation factor IXa). These two interactions were monitored on surfaces modified with biotin-streptavidinbiotin and N-(2- trifluoroethanesulfonatoethyl)-N-(methyl)triethoxysilylpropyl-3-amine, respectively.

    Original languageEnglish
    Pages (from-to)6602-6609
    Number of pages8
    JournalAnalytical Chemistry
    Volume80
    Issue number17
    DOIs
    Publication statusPublished - 2008 Sep 1

    Fingerprint

    Nucleotide Aptamers
    Nanosensors
    Labels
    Waveguides
    Evanescent fields
    Ligands
    Sensors
    Vitamin B 12
    Factor IXa
    RNA
    Biomolecules
    Biotin
    Refraction
    Nucleic Acids
    Proteins
    Adsorption
    Molecules

    ASJC Scopus subject areas

    • Analytical Chemistry

    Cite this

    Influence of nanometric holes on the sensitivity of a waveguide-mode sensor : Label-free nanosensor for the analysis of RNA aptamer-ligand interactions. / Gopinath, Subash C B; Awazu, Koichi; Fujimaki, Makoto; Sugimoto, Katsuaki; Ohki, Yoshimichi; Komatsubara, Tetsuro; Tominaga, Junji; Gupta, Kailash C.; Kumar, Penmetcha K R.

    In: Analytical Chemistry, Vol. 80, No. 17, 01.09.2008, p. 6602-6609.

    Research output: Contribution to journalArticle

    Gopinath, SCB, Awazu, K, Fujimaki, M, Sugimoto, K, Ohki, Y, Komatsubara, T, Tominaga, J, Gupta, KC & Kumar, PKR 2008, 'Influence of nanometric holes on the sensitivity of a waveguide-mode sensor: Label-free nanosensor for the analysis of RNA aptamer-ligand interactions', Analytical Chemistry, vol. 80, no. 17, pp. 6602-6609. https://doi.org/10.1021/ac800767s
    Gopinath, Subash C B ; Awazu, Koichi ; Fujimaki, Makoto ; Sugimoto, Katsuaki ; Ohki, Yoshimichi ; Komatsubara, Tetsuro ; Tominaga, Junji ; Gupta, Kailash C. ; Kumar, Penmetcha K R. / Influence of nanometric holes on the sensitivity of a waveguide-mode sensor : Label-free nanosensor for the analysis of RNA aptamer-ligand interactions. In: Analytical Chemistry. 2008 ; Vol. 80, No. 17. pp. 6602-6609.
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