Influence of nanoparticle-embedded polymeric surfaces on cellular adhesion, proliferation, and differentiation

Letizia Ventrelli, Toshinori Fujie, Serena Del Turco, Giuseppina Basta, Barbara Mazzolai, Virgilio Mattoli

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The development of functional substrates to direct cellular organization is important for biomedical applications such as regenerative medicine and biorobotics. In this study, we prepared freestanding polymeric ultrathin films (nanofilms) consisting of poly(lactic acid) (PLA) and magnetic nanoparticles (MNPs), and evaluated the effects of their surface properties on the organization of cardiac-like rat myoblasts (H9c2). We changed surface properties of the PLA nanofilms (i.e., roughness and wettability) as a function of MNPs concentration. We found that the incorporation of MNPs into the nanofilms enhanced both proliferation and adhesion of H9c2 cells. Through the morphological assessment of the differentiated H9c2 cells, we also found that the presence of MNPs significantly increased the fusion index and the surface area of myotubes. In conclusion, the embedding of MNPs is a simple method to tailor the physicochemical properties of the polymeric nanofilms, yet it is an effective approach to enhance the cellular morphogenesis in the field of cardiac tissue engineering for regenerative medicine and biorobotics applications.

Original languageEnglish
Pages (from-to)2652-2661
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume102
Issue number8
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Nanoparticles
Adhesion
Cell Proliferation
Regenerative Medicine
Surface Properties
Surface properties
Wettability
Ultrathin films
Myoblasts
Skeletal Muscle Fibers
Lactic acid
Tissue Engineering
Morphogenesis
Tissue engineering
Polymer films
Cell Adhesion
Wetting
Rats
Fusion reactions
Surface roughness

Keywords

  • cardiac tissue engineering
  • cellular scaffolds
  • magnetic nanoparticles
  • surface roughness
  • ultrathin films (nanofilms)

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys
  • Medicine(all)

Cite this

Influence of nanoparticle-embedded polymeric surfaces on cellular adhesion, proliferation, and differentiation. / Ventrelli, Letizia; Fujie, Toshinori; Turco, Serena Del; Basta, Giuseppina; Mazzolai, Barbara; Mattoli, Virgilio.

In: Journal of Biomedical Materials Research - Part A, Vol. 102, No. 8, 2014, p. 2652-2661.

Research output: Contribution to journalArticle

Ventrelli, Letizia ; Fujie, Toshinori ; Turco, Serena Del ; Basta, Giuseppina ; Mazzolai, Barbara ; Mattoli, Virgilio. / Influence of nanoparticle-embedded polymeric surfaces on cellular adhesion, proliferation, and differentiation. In: Journal of Biomedical Materials Research - Part A. 2014 ; Vol. 102, No. 8. pp. 2652-2661.
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