Abstract
We investigated the influence of a single exhaustive bout of downhill running on oxidative damage to DNA and changes of antioxidant vitamin concentrations in rats. Plasma vitamin E levels were unchanged up to 48 hr postexercise. However, plasma ascorbic acid (AA) levels increased after the exercise, then decreased thereafter. This increase corresponded to a marked decrease in AA concentration in the adrenal glands. The activity of hepatic l-gulono-γ-lactone oxidase, which catalyzes AA synthesis, was unaltered after the exercise. The weight of the adrenal glands was significantly increased 24 hr postexercise. These results indicate that the change in the plasma AA concentration after vigorous exercise was due mainly to the release of AA from the adrenal glands. The plasma creatine phosphokinase (CPK) activity and white blood cell (WBC) count increased 3 to 6 hr postexercise. Over this same period, a marker of oxidative DNA damage, 8-hydroxydeoxyguanosine in DNA, increased in the WBC, but not in the foreleg muscle. Lipid peroxide and vitamin E levels were also unchanged in the foreleg muscle. There was a positive correlation between CPK activity in the plasma and DNA damage in the WBC, suggesting that the DNA damage in the WBC was closely related with muscle damage due to exercise. Copyright (C) 2000 Elsevier Science Inc.
| Original language | English |
|---|---|
| Pages (from-to) | 401-407 |
| Number of pages | 7 |
| Journal | Journal of Nutritional Biochemistry |
| Volume | 11 |
| Issue number | 7-8 |
| DOIs | |
| Publication status | Published - 2000 |
| Externally published | Yes |
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Keywords
- 8-hydroxydeoxyguanosine
- Ascorbic acid
- DNA damage
- Stress
- White blood cells
ASJC Scopus subject areas
- Biochemistry
- Endocrinology, Diabetes and Metabolism
Cite this
Influence of one bout of vigorous exercise on ascorbic acid in plasma and oxidative damage to DNA in blood cells and muscle in untrained rats. / Umegaki, Keizo; Daohua, Pang; Sugisawa, Ayako; Kimura, Michiyo; Higuchi, Mitsuru.
In: Journal of Nutritional Biochemistry, Vol. 11, No. 7-8, 2000, p. 401-407.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Influence of one bout of vigorous exercise on ascorbic acid in plasma and oxidative damage to DNA in blood cells and muscle in untrained rats
AU - Umegaki, Keizo
AU - Daohua, Pang
AU - Sugisawa, Ayako
AU - Kimura, Michiyo
AU - Higuchi, Mitsuru
PY - 2000
Y1 - 2000
N2 - We investigated the influence of a single exhaustive bout of downhill running on oxidative damage to DNA and changes of antioxidant vitamin concentrations in rats. Plasma vitamin E levels were unchanged up to 48 hr postexercise. However, plasma ascorbic acid (AA) levels increased after the exercise, then decreased thereafter. This increase corresponded to a marked decrease in AA concentration in the adrenal glands. The activity of hepatic l-gulono-γ-lactone oxidase, which catalyzes AA synthesis, was unaltered after the exercise. The weight of the adrenal glands was significantly increased 24 hr postexercise. These results indicate that the change in the plasma AA concentration after vigorous exercise was due mainly to the release of AA from the adrenal glands. The plasma creatine phosphokinase (CPK) activity and white blood cell (WBC) count increased 3 to 6 hr postexercise. Over this same period, a marker of oxidative DNA damage, 8-hydroxydeoxyguanosine in DNA, increased in the WBC, but not in the foreleg muscle. Lipid peroxide and vitamin E levels were also unchanged in the foreleg muscle. There was a positive correlation between CPK activity in the plasma and DNA damage in the WBC, suggesting that the DNA damage in the WBC was closely related with muscle damage due to exercise. Copyright (C) 2000 Elsevier Science Inc.
AB - We investigated the influence of a single exhaustive bout of downhill running on oxidative damage to DNA and changes of antioxidant vitamin concentrations in rats. Plasma vitamin E levels were unchanged up to 48 hr postexercise. However, plasma ascorbic acid (AA) levels increased after the exercise, then decreased thereafter. This increase corresponded to a marked decrease in AA concentration in the adrenal glands. The activity of hepatic l-gulono-γ-lactone oxidase, which catalyzes AA synthesis, was unaltered after the exercise. The weight of the adrenal glands was significantly increased 24 hr postexercise. These results indicate that the change in the plasma AA concentration after vigorous exercise was due mainly to the release of AA from the adrenal glands. The plasma creatine phosphokinase (CPK) activity and white blood cell (WBC) count increased 3 to 6 hr postexercise. Over this same period, a marker of oxidative DNA damage, 8-hydroxydeoxyguanosine in DNA, increased in the WBC, but not in the foreleg muscle. Lipid peroxide and vitamin E levels were also unchanged in the foreleg muscle. There was a positive correlation between CPK activity in the plasma and DNA damage in the WBC, suggesting that the DNA damage in the WBC was closely related with muscle damage due to exercise. Copyright (C) 2000 Elsevier Science Inc.
KW - 8-hydroxydeoxyguanosine
KW - Ascorbic acid
KW - DNA damage
KW - Stress
KW - White blood cells
UR - http://www.scopus.com/inward/record.url?scp=0033820864&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033820864&partnerID=8YFLogxK
U2 - 10.1016/S0955-2863(00)00096-6
DO - 10.1016/S0955-2863(00)00096-6
M3 - Article
VL - 11
SP - 401
EP - 407
JO - Journal of Nutritional Biochemistry
JF - Journal of Nutritional Biochemistry
SN - 0955-2863
IS - 7-8
ER -