Influence of Porphyromonas gingivalis in gut microbiota of streptozotocin-induced diabetic mice

Anri Ohtsu, Yasuo Takeuchi, Sayaka Katagiri, Wataru Suda, Shogo Maekawa, Takahiko Shiba, Rina Komazaki, Sayuri Udagawa, Naoki Sasaki, Masahira Hattori, Yuichi Izumi

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Objectives: Increasing evidence suggests that periodontitis can exacerbate diabetes, and gut bacterial dysbiosis appears to be linked with the diabetic condition. The present study examined the effects of oral administration of the periodontopathic bacterium, Porphyromonas gingivalis, on the gut microbiota and systemic conditions in streptozotocin-induced diabetic mice. Materials and Methods: Diabetes was induced by streptozotocin injection in C57BL/6J male mice (STZ). STZ and wild-type (WT) mice were orally administered P. gingivalis (STZPg, WTPg) or saline (STZco, WTco). Feces were collected, and the gut microbiome was examined by 16S rRNA gene sequencing. The expression of genes related to inflammation, epithelial tight junctions, and glucose/fatty acid metabolism in the ileum or liver were examined by quantitative PCR. Results: The relative abundance of several genera, including Brevibacterium, Corynebacterium, and Facklamia, was significantly increased in STZco mice compared to WTco mice. The relative abundances of Staphylococcus and Turicibacter in the gut microbiome were altered by oral administration of P. gingivalis in STZ mice. STZPg mice showed higher concentrations of fasting blood glucose and inflammatory genes levels in the ileum, compared to STZco mice. Conclusions: Oral administration of P. gingivalis altered the gut microbiota and aggravated glycemic control in streptozotocin-induced diabetic mice.

    Original languageEnglish
    JournalOral Diseases
    DOIs
    Publication statusPublished - 2019 Jan 1

    Fingerprint

    Porphyromonas gingivalis
    Streptozocin
    Oral Administration
    Ileum
    Dysbiosis
    Brevibacterium
    Gastrointestinal Microbiome
    Corynebacterium
    Tight Junctions
    Periodontitis
    Staphylococcus
    rRNA Genes
    Feces
    Blood Glucose
    Fasting
    Fatty Acids
    Inflammation
    Bacteria
    Gene Expression
    Glucose

    Keywords

    • gut microbiota
    • periodontitis
    • streptozotocin

    ASJC Scopus subject areas

    • Otorhinolaryngology
    • Dentistry(all)

    Cite this

    Ohtsu, A., Takeuchi, Y., Katagiri, S., Suda, W., Maekawa, S., Shiba, T., ... Izumi, Y. (2019). Influence of Porphyromonas gingivalis in gut microbiota of streptozotocin-induced diabetic mice. Oral Diseases. https://doi.org/10.1111/odi.13044

    Influence of Porphyromonas gingivalis in gut microbiota of streptozotocin-induced diabetic mice. / Ohtsu, Anri; Takeuchi, Yasuo; Katagiri, Sayaka; Suda, Wataru; Maekawa, Shogo; Shiba, Takahiko; Komazaki, Rina; Udagawa, Sayuri; Sasaki, Naoki; Hattori, Masahira; Izumi, Yuichi.

    In: Oral Diseases, 01.01.2019.

    Research output: Contribution to journalArticle

    Ohtsu, A, Takeuchi, Y, Katagiri, S, Suda, W, Maekawa, S, Shiba, T, Komazaki, R, Udagawa, S, Sasaki, N, Hattori, M & Izumi, Y 2019, 'Influence of Porphyromonas gingivalis in gut microbiota of streptozotocin-induced diabetic mice', Oral Diseases. https://doi.org/10.1111/odi.13044
    Ohtsu, Anri ; Takeuchi, Yasuo ; Katagiri, Sayaka ; Suda, Wataru ; Maekawa, Shogo ; Shiba, Takahiko ; Komazaki, Rina ; Udagawa, Sayuri ; Sasaki, Naoki ; Hattori, Masahira ; Izumi, Yuichi. / Influence of Porphyromonas gingivalis in gut microbiota of streptozotocin-induced diabetic mice. In: Oral Diseases. 2019.
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    author = "Anri Ohtsu and Yasuo Takeuchi and Sayaka Katagiri and Wataru Suda and Shogo Maekawa and Takahiko Shiba and Rina Komazaki and Sayuri Udagawa and Naoki Sasaki and Masahira Hattori and Yuichi Izumi",
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    AU - Takeuchi, Yasuo

    AU - Katagiri, Sayaka

    AU - Suda, Wataru

    AU - Maekawa, Shogo

    AU - Shiba, Takahiko

    AU - Komazaki, Rina

    AU - Udagawa, Sayuri

    AU - Sasaki, Naoki

    AU - Hattori, Masahira

    AU - Izumi, Yuichi

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    AB - Objectives: Increasing evidence suggests that periodontitis can exacerbate diabetes, and gut bacterial dysbiosis appears to be linked with the diabetic condition. The present study examined the effects of oral administration of the periodontopathic bacterium, Porphyromonas gingivalis, on the gut microbiota and systemic conditions in streptozotocin-induced diabetic mice. Materials and Methods: Diabetes was induced by streptozotocin injection in C57BL/6J male mice (STZ). STZ and wild-type (WT) mice were orally administered P. gingivalis (STZPg, WTPg) or saline (STZco, WTco). Feces were collected, and the gut microbiome was examined by 16S rRNA gene sequencing. The expression of genes related to inflammation, epithelial tight junctions, and glucose/fatty acid metabolism in the ileum or liver were examined by quantitative PCR. Results: The relative abundance of several genera, including Brevibacterium, Corynebacterium, and Facklamia, was significantly increased in STZco mice compared to WTco mice. The relative abundances of Staphylococcus and Turicibacter in the gut microbiome were altered by oral administration of P. gingivalis in STZ mice. STZPg mice showed higher concentrations of fasting blood glucose and inflammatory genes levels in the ileum, compared to STZco mice. Conclusions: Oral administration of P. gingivalis altered the gut microbiota and aggravated glycemic control in streptozotocin-induced diabetic mice.

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