Influence of temperature on lactate utilization by round spermatids from rat testes

M. Nakamura, I. Yasumasu, S. Okinaga, K. Arai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Rotenone-sensitive 14CO2 formation from [14C]lactate and oxygen consumption by round spermatids were found to be greater at elevated temperatures than at 34°C. More than 96% of the total radioactivity of the metabolized [14C]lactate was recovered in the released CO2 and the acid soluble fraction of the cells. There was practically no incorporation of [14C]lactate into the lipid, nucleic acid, and protein fractions. Intracellular level of ATP in spermatids was enhanced in the presence of lactate (20 mM) at 34°C (scrotal temperature), whereas it was decreased at 37°C (body temperature). However, this was reversible when the cells were transferred from the elevated temperature to 34°C. It was also found that oxygen consumption and CO2 production were increased at 34° C by 2,4-dinitrophenol (DNP), but decreased by oligomycin. On the contrary, oligomycin and DNP had no effect on oxygen consumption and 14CO2 formation at the elevated temperature. These findings provide evidence that lactate utilization by spermatids is coupled with oxidative phosphorylation at scrotal temperature, but becomes uncoupled at elevated temperature, although more lactate is consumed.

Original languageEnglish
Pages (from-to)265-272
Number of pages8
JournalDevelopment Growth and Differentiation
Volume24
Issue number3
Publication statusPublished - 1982
Externally publishedYes

Fingerprint

Spermatids
Testis
Lactic Acid
Temperature
Oxygen Consumption
Oligomycins
Dinitrophenols
2,4-Dinitrophenol
Rotenone
Oxidative Phosphorylation
Body Temperature
Radioactivity
Nucleic Acids
Adenosine Triphosphate
Lipids
Acids
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Anatomy

Cite this

Influence of temperature on lactate utilization by round spermatids from rat testes. / Nakamura, M.; Yasumasu, I.; Okinaga, S.; Arai, K.

In: Development Growth and Differentiation, Vol. 24, No. 3, 1982, p. 265-272.

Research output: Contribution to journalArticle

Nakamura, M, Yasumasu, I, Okinaga, S & Arai, K 1982, 'Influence of temperature on lactate utilization by round spermatids from rat testes', Development Growth and Differentiation, vol. 24, no. 3, pp. 265-272.
Nakamura, M. ; Yasumasu, I. ; Okinaga, S. ; Arai, K. / Influence of temperature on lactate utilization by round spermatids from rat testes. In: Development Growth and Differentiation. 1982 ; Vol. 24, No. 3. pp. 265-272.
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