Influence of the redox potential of the primary quinone electron acceptor on photoinhibition in photosystem II

Christian Fufezan, Christine M. Gross, Martin Sjödin, A. William Rutherford, Anja Krieger-Liszkay, Diana Kirilovsky

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

We report the characterization of the effects of the A249S mutation located within the binding pocket of the primary quinone electron acceptor, Q A, in the D2 subunit of photosystem II in Thermosynechococcus elongatus. This mutation shifts the redox potential of QA by ∼-60 mV. This mutant provides an opportunity to test the hypothesis, proposed earlier from herbicide-induced redox effects, that photoinhibition (light-induced damage of the photosynthetic apparatus) is modulated by the potential of QA. Thus the influence of the redox potential of Q A on photoinhibition was investigated in vivo and in vitro. Compared with the wild-type, the A249S mutant showed an accelerated photoinhibition and an increase in singlet oxygen production. Measurements of thermoluminescence and of the fluorescence yield decay kinetics indicated that the charge-separated state involving QA was destabilized in the A249S mutant. These findings support the hypothesis that a decrease in the redox potential of Q A causes an increase in singlet oxygen-mediated photoinhibition by favoring the back-reaction route that involves formation of the reaction center chlorophyll triplet. The kinetics of charge recombination are interpreted in terms of a dynamic structural heterogeneity in photosystem II that results in high and low potential forms of QA. The effect of the A249S mutation seems to reflect a shift in the structural equilibrium favoring the low potential form.

Original languageEnglish
Pages (from-to)12492-12502
Number of pages11
JournalJournal of Biological Chemistry
Volume282
Issue number17
DOIs
Publication statusPublished - 2007 Apr 27
Externally publishedYes

Fingerprint

Photosystem II Protein Complex
Oxidation-Reduction
Electrons
Singlet Oxygen
Mutation
Kinetics
Thermoluminescence
Structural dynamics
Herbicides
Chlorophyll
Genetic Recombination
Fluorescence
Light
benzoquinone

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Fufezan, C., Gross, C. M., Sjödin, M., Rutherford, A. W., Krieger-Liszkay, A., & Kirilovsky, D. (2007). Influence of the redox potential of the primary quinone electron acceptor on photoinhibition in photosystem II. Journal of Biological Chemistry, 282(17), 12492-12502. https://doi.org/10.1074/jbc.M610951200

Influence of the redox potential of the primary quinone electron acceptor on photoinhibition in photosystem II. / Fufezan, Christian; Gross, Christine M.; Sjödin, Martin; Rutherford, A. William; Krieger-Liszkay, Anja; Kirilovsky, Diana.

In: Journal of Biological Chemistry, Vol. 282, No. 17, 27.04.2007, p. 12492-12502.

Research output: Contribution to journalArticle

Fufezan, C, Gross, CM, Sjödin, M, Rutherford, AW, Krieger-Liszkay, A & Kirilovsky, D 2007, 'Influence of the redox potential of the primary quinone electron acceptor on photoinhibition in photosystem II', Journal of Biological Chemistry, vol. 282, no. 17, pp. 12492-12502. https://doi.org/10.1074/jbc.M610951200
Fufezan, Christian ; Gross, Christine M. ; Sjödin, Martin ; Rutherford, A. William ; Krieger-Liszkay, Anja ; Kirilovsky, Diana. / Influence of the redox potential of the primary quinone electron acceptor on photoinhibition in photosystem II. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 17. pp. 12492-12502.
@article{9d465b246ed64a179b39dbb8ca1350da,
title = "Influence of the redox potential of the primary quinone electron acceptor on photoinhibition in photosystem II",
abstract = "We report the characterization of the effects of the A249S mutation located within the binding pocket of the primary quinone electron acceptor, Q A, in the D2 subunit of photosystem II in Thermosynechococcus elongatus. This mutation shifts the redox potential of QA by ∼-60 mV. This mutant provides an opportunity to test the hypothesis, proposed earlier from herbicide-induced redox effects, that photoinhibition (light-induced damage of the photosynthetic apparatus) is modulated by the potential of QA. Thus the influence of the redox potential of Q A on photoinhibition was investigated in vivo and in vitro. Compared with the wild-type, the A249S mutant showed an accelerated photoinhibition and an increase in singlet oxygen production. Measurements of thermoluminescence and of the fluorescence yield decay kinetics indicated that the charge-separated state involving QA was destabilized in the A249S mutant. These findings support the hypothesis that a decrease in the redox potential of Q A causes an increase in singlet oxygen-mediated photoinhibition by favoring the back-reaction route that involves formation of the reaction center chlorophyll triplet. The kinetics of charge recombination are interpreted in terms of a dynamic structural heterogeneity in photosystem II that results in high and low potential forms of QA. The effect of the A249S mutation seems to reflect a shift in the structural equilibrium favoring the low potential form.",
author = "Christian Fufezan and Gross, {Christine M.} and Martin Sj{\"o}din and Rutherford, {A. William} and Anja Krieger-Liszkay and Diana Kirilovsky",
year = "2007",
month = "4",
day = "27",
doi = "10.1074/jbc.M610951200",
language = "English",
volume = "282",
pages = "12492--12502",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "17",

}

TY - JOUR

T1 - Influence of the redox potential of the primary quinone electron acceptor on photoinhibition in photosystem II

AU - Fufezan, Christian

AU - Gross, Christine M.

AU - Sjödin, Martin

AU - Rutherford, A. William

AU - Krieger-Liszkay, Anja

AU - Kirilovsky, Diana

PY - 2007/4/27

Y1 - 2007/4/27

N2 - We report the characterization of the effects of the A249S mutation located within the binding pocket of the primary quinone electron acceptor, Q A, in the D2 subunit of photosystem II in Thermosynechococcus elongatus. This mutation shifts the redox potential of QA by ∼-60 mV. This mutant provides an opportunity to test the hypothesis, proposed earlier from herbicide-induced redox effects, that photoinhibition (light-induced damage of the photosynthetic apparatus) is modulated by the potential of QA. Thus the influence of the redox potential of Q A on photoinhibition was investigated in vivo and in vitro. Compared with the wild-type, the A249S mutant showed an accelerated photoinhibition and an increase in singlet oxygen production. Measurements of thermoluminescence and of the fluorescence yield decay kinetics indicated that the charge-separated state involving QA was destabilized in the A249S mutant. These findings support the hypothesis that a decrease in the redox potential of Q A causes an increase in singlet oxygen-mediated photoinhibition by favoring the back-reaction route that involves formation of the reaction center chlorophyll triplet. The kinetics of charge recombination are interpreted in terms of a dynamic structural heterogeneity in photosystem II that results in high and low potential forms of QA. The effect of the A249S mutation seems to reflect a shift in the structural equilibrium favoring the low potential form.

AB - We report the characterization of the effects of the A249S mutation located within the binding pocket of the primary quinone electron acceptor, Q A, in the D2 subunit of photosystem II in Thermosynechococcus elongatus. This mutation shifts the redox potential of QA by ∼-60 mV. This mutant provides an opportunity to test the hypothesis, proposed earlier from herbicide-induced redox effects, that photoinhibition (light-induced damage of the photosynthetic apparatus) is modulated by the potential of QA. Thus the influence of the redox potential of Q A on photoinhibition was investigated in vivo and in vitro. Compared with the wild-type, the A249S mutant showed an accelerated photoinhibition and an increase in singlet oxygen production. Measurements of thermoluminescence and of the fluorescence yield decay kinetics indicated that the charge-separated state involving QA was destabilized in the A249S mutant. These findings support the hypothesis that a decrease in the redox potential of Q A causes an increase in singlet oxygen-mediated photoinhibition by favoring the back-reaction route that involves formation of the reaction center chlorophyll triplet. The kinetics of charge recombination are interpreted in terms of a dynamic structural heterogeneity in photosystem II that results in high and low potential forms of QA. The effect of the A249S mutation seems to reflect a shift in the structural equilibrium favoring the low potential form.

UR - http://www.scopus.com/inward/record.url?scp=34250332693&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250332693&partnerID=8YFLogxK

U2 - 10.1074/jbc.M610951200

DO - 10.1074/jbc.M610951200

M3 - Article

C2 - 17327225

AN - SCOPUS:34250332693

VL - 282

SP - 12492

EP - 12502

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 17

ER -