Infrared reflection absorption and sum frequency generation spectroscopic study on the structures of the LB films of palmitoyl-L- and DL-ornithine and palmitoyl-L- and DL-lysine

K. Itoh, C. Ohe, T. Tsurumaru, S. Yasukawa, Tadashi Yamaguchi, G. Kasuya

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Infrared reflection absorption (IRA) were measured for the Langmuir-Blodgett (LB) films of palmitoyl-lysine (PL) and palmitoyl-ornithine (PO) with various mixing ratios of the L- and D-enantiomers. The introduction of the D-enantiomers to the LB films consisting of the L-enantiomers in PO-LB does not cause any frequency shifts of the amide I and II bands of the peptide groups in PO-LB, while the introduction causes appreciable frequency changes in PL-LB. The IRA spectra of the LB films soaked in D2O indicated a much higher H/D exchange rate of the peptide groups for PL-LB than that for PO-LB. These results proved the existence of a more flexible molecular assembly in PL-LB compared to that in PO-LB. The SFG spectrum of PL-LB (D/L = 0/10) gives rise to peaks at 3220 and 3160cm-1, which are absent in the SFG spectra of PO-LB (D/L = 0/10). The peaks are ascribable to water molecules in an ordered arrangement, which play as a channel for the H/D exchange process. The bound water molecules render PL-LB more hydrophilic than PO-LB, which explains the fact that the PL-LB forms a Z-type structure and PO-LB a Y-type structure.

    Original languageEnglish
    Pages (from-to)197-203
    Number of pages7
    JournalVibrational Spectroscopy
    Volume29
    Issue number1-2
    DOIs
    Publication statusPublished - 2002 Jul 5

    Fingerprint

    Ornithine
    Langmuir Blodgett films
    Lysine
    Infrared radiation
    Enantiomers
    Peptides
    Molecules
    Water
    Amides
    Absorption spectra

    Keywords

    • Infrared reflection absorption
    • Langmuir-Blodgett films
    • Palmitoyl-lysine (PL) and palmitoyl-ornithine (PO)

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Spectroscopy

    Cite this

    Infrared reflection absorption and sum frequency generation spectroscopic study on the structures of the LB films of palmitoyl-L- and DL-ornithine and palmitoyl-L- and DL-lysine. / Itoh, K.; Ohe, C.; Tsurumaru, T.; Yasukawa, S.; Yamaguchi, Tadashi; Kasuya, G.

    In: Vibrational Spectroscopy, Vol. 29, No. 1-2, 05.07.2002, p. 197-203.

    Research output: Contribution to journalArticle

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    abstract = "Infrared reflection absorption (IRA) were measured for the Langmuir-Blodgett (LB) films of palmitoyl-lysine (PL) and palmitoyl-ornithine (PO) with various mixing ratios of the L- and D-enantiomers. The introduction of the D-enantiomers to the LB films consisting of the L-enantiomers in PO-LB does not cause any frequency shifts of the amide I and II bands of the peptide groups in PO-LB, while the introduction causes appreciable frequency changes in PL-LB. The IRA spectra of the LB films soaked in D2O indicated a much higher H/D exchange rate of the peptide groups for PL-LB than that for PO-LB. These results proved the existence of a more flexible molecular assembly in PL-LB compared to that in PO-LB. The SFG spectrum of PL-LB (D/L = 0/10) gives rise to peaks at 3220 and 3160cm-1, which are absent in the SFG spectra of PO-LB (D/L = 0/10). The peaks are ascribable to water molecules in an ordered arrangement, which play as a channel for the H/D exchange process. The bound water molecules render PL-LB more hydrophilic than PO-LB, which explains the fact that the PL-LB forms a Z-type structure and PO-LB a Y-type structure.",
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