Inhibition by 5-thio-d-glucopyranose of protein biosynthesis in vitro in spermatids from RAT testis

Masahisa Nakamura, Peter F. Hall

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The effect of 5-thio-d-glucopyranose (thioglucose) upon protein biosynthesis in vitro was examined in testes from mature rats. Thioglucose in vitro is without demonstrable effect upon incorporation of l-[U-14C]phenylalanine into protein by whole testis but inhibits this incorporation by a purified fraction of immature spermatids (stages 1-8) prepared by centrifugal elutriation; the inhibition is observed with or without glucose added in vitro and is concentration dependent in the range 1-50 mM. Similar inhibition is observed with three other 14C-labeled amino acids (leucine, lysine and glutamate). Mature spermatids (>stage 8) and other heterogeneous fractions of testicular cells prepared by the same method also show inhibition by thioglucose of incorporation of phenylalanine into protein so that it is not possible to say that the effect is confined to spermatids although it is most pronounced in these cells. Inhibition of protein synthesis in vitro is also observed when thioglucose was administered in vivo (33 mg/kg body wt./day). This change occurs at the minimal dose observed by other workers to produce arrest of spermatogenesis and hence infertility.

Original languageEnglish
Pages (from-to)474-483
Number of pages10
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume447
Issue number4
DOIs
Publication statusPublished - 1976 Nov 1
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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