Inhibitor and temperature effect on catalase in the liver of adult diploid and haploid Rana rugosa

Akihiko Kashiwagi, Keiko Kashiwagi, Minoru Takase, Hideki Hanada, Masamichi Yamashita, Tomio Naitoh, Masahisa Nakamura

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The authors succeeded in raising a single mature haploid Rana rugosa female to the age of 2 years from an egg artificially fertilized with ultraviolet irradiated sperm. In order to discover why this particular haploid individual should survive so long, hydrogen peroxide detoxifying catalase in the liver of this individual and age matched diploids was examined and compared for total activity, temperature stability, and chemical inhibition. Total activity was found to be significantly higher in the haploid frog than in the diploids, suggesting that this particular haploid had a unique system for hydrogen peroxide detoxification which protected the liver against cell death, preventing hepatic failure, and leading to a prolonged survival. Liver catalase from the haploid proved to be more labile to aminotriazole and urea, losing 60-70% of its original activity after 30 min treatment, whereas diploid catalase lost only 40% under the same conditions. Haploid and diploid catalase responded similarly to heat, however. It seems likely that inhibitor-binding sites differ considerably between the catalase of normal diploids and the catalase of this particular haploid, while overall structure is generally similar.

Original languageEnglish
Pages (from-to)235-239
Number of pages5
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume119
Issue number1
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Ranidae
Haploidy
Diploidy
Liver
Thermal effects
Catalase
Temperature
Hydrogen Peroxide
Amitrole
Detoxification
Cell death
Zygote
Liver Failure
Urea
Anura
Spermatozoa
Binding Sites
Cell Death
Hot Temperature

Keywords

  • Catalase activity
  • Catalase chemical inhibition
  • Catalase temperature stability
  • HO removal mechanisms
  • Haploid survival
  • Haploid syndrome
  • Peroxisomal enzymes
  • Programmed cell death
  • Rana rugosa

ASJC Scopus subject areas

  • Biochemistry
  • Physiology

Cite this

Inhibitor and temperature effect on catalase in the liver of adult diploid and haploid Rana rugosa. / Kashiwagi, Akihiko; Kashiwagi, Keiko; Takase, Minoru; Hanada, Hideki; Yamashita, Masamichi; Naitoh, Tomio; Nakamura, Masahisa.

In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 119, No. 1, 1998, p. 235-239.

Research output: Contribution to journalArticle

Kashiwagi, Akihiko ; Kashiwagi, Keiko ; Takase, Minoru ; Hanada, Hideki ; Yamashita, Masamichi ; Naitoh, Tomio ; Nakamura, Masahisa. / Inhibitor and temperature effect on catalase in the liver of adult diploid and haploid Rana rugosa. In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 1998 ; Vol. 119, No. 1. pp. 235-239.
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