Inhibitory effect of flavonoids on DNA-dependent DNA and RNA polymerases

K. Shinozuka, Yo Kikuchi, C. Nishino, A. Mori, S. Tawata

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Flavonoids, (-)-epigallocatechin (1), myricetin (2) and quercetin (3), were investigated for inhibitory effects on E. coli DNA polymerase I and T7 bacteriophage RNA polymerase. In both DNA and RNA synthesis, 1 and 3 inhibited enzyme reactions by non-competitive and mixed type inhibition respecitively, with regard to template DNAs. Myricetin (2) inhibited DNA and RNA polymerase reactions by mixed type and competitive type inhibition, respectively, with template DNAs. It was suggested that 2 interacts with covalently closed/circular DNA.

Original languageEnglish
Pages (from-to)882-885
Number of pages4
JournalExperientia
Volume44
Issue number10
DOIs
Publication statusPublished - 1988 Oct
Externally publishedYes

Keywords

  • DNA polymerase
  • Flavonoids
  • inhibitory effect
  • RNA polymerase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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