Inhibitory effects of oleic and docosahexaenoic acids on lung metastasis by colon-carcinoma-26 cells are associated with reduced matrix metalloproteinase-2 and -9 activities

Izuru Suzuki, Masaaki Iigo, Chikako Ishikawa, Tetsuya Kuhara, Makoto Asamoto, Takehiko Kunimoto, Malcolm A. Moore, Kazunaga Yazawa, Eiji Araki, Hiroyuki Tsuda

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

In order to determine the effects of single unsaturated fatty acids (UFAs) or combinations on establishment of lung metastatic colonies, UFAs were administered orally to CDF1 mice bearing s.c. implants of the highly metastatic colon carcinoma 26. Oleic acid (OA), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) demonstrated significant inhibition. In the case of DHA, this inhibitory potential was markedly reduced by co- administration of linoleic acid (LA) or EPA. Furthermore, while tumor cells treated with DHA showed a very low potential for lung colony formation when injected i.v., this again being partially reversed by coadministration of EPA. UFAs were found to be well absorbed into tumor tissues after oral administration, causing marked changes in relative levels, the arachidonic acid (AA) content, in particular, being markedly decreased by treatment with DHA or EPA, but not with DHA plus EPA or with DHA plus LA. Investigation of the gelatinolytic activity of the 57-kDa and 92-kDa isoforms of type-IV collagenase (MMP-2 and MMP.9, respectively) showed a clear reduction in the former by treatment with OA, while DHA, but not DHA plus LA or EPA, caused a decrease in the 92-kDa isoform, which was well correlated with AA content in tumor tissues (r = 0.900, p < 0.001). These results suggest that inhibition of metastasis due to treatment with OA and DHA might be due to depressed type-IV collagenase activity.

Original languageEnglish
Pages (from-to)607-612
Number of pages6
JournalInternational Journal of Cancer
Volume73
Issue number4
DOIs
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Oleic Acids
Docosahexaenoic Acids
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Colon
Eicosapentaenoic Acid
Neoplasm Metastasis
Carcinoma
Lung
Linoleic Acid
Oleic Acid
Unsaturated Fatty Acids
Arachidonic Acid
Protein Isoforms
Matrix Metalloproteinase 8
Neoplasms
Collagenases
Matrix Metalloproteinases
Oral Administration
Therapeutics

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Inhibitory effects of oleic and docosahexaenoic acids on lung metastasis by colon-carcinoma-26 cells are associated with reduced matrix metalloproteinase-2 and -9 activities. / Suzuki, Izuru; Iigo, Masaaki; Ishikawa, Chikako; Kuhara, Tetsuya; Asamoto, Makoto; Kunimoto, Takehiko; Moore, Malcolm A.; Yazawa, Kazunaga; Araki, Eiji; Tsuda, Hiroyuki.

In: International Journal of Cancer, Vol. 73, No. 4, 1997, p. 607-612.

Research output: Contribution to journalArticle

Suzuki, Izuru ; Iigo, Masaaki ; Ishikawa, Chikako ; Kuhara, Tetsuya ; Asamoto, Makoto ; Kunimoto, Takehiko ; Moore, Malcolm A. ; Yazawa, Kazunaga ; Araki, Eiji ; Tsuda, Hiroyuki. / Inhibitory effects of oleic and docosahexaenoic acids on lung metastasis by colon-carcinoma-26 cells are associated with reduced matrix metalloproteinase-2 and -9 activities. In: International Journal of Cancer. 1997 ; Vol. 73, No. 4. pp. 607-612.
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abstract = "In order to determine the effects of single unsaturated fatty acids (UFAs) or combinations on establishment of lung metastatic colonies, UFAs were administered orally to CDF1 mice bearing s.c. implants of the highly metastatic colon carcinoma 26. Oleic acid (OA), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) demonstrated significant inhibition. In the case of DHA, this inhibitory potential was markedly reduced by co- administration of linoleic acid (LA) or EPA. Furthermore, while tumor cells treated with DHA showed a very low potential for lung colony formation when injected i.v., this again being partially reversed by coadministration of EPA. UFAs were found to be well absorbed into tumor tissues after oral administration, causing marked changes in relative levels, the arachidonic acid (AA) content, in particular, being markedly decreased by treatment with DHA or EPA, but not with DHA plus EPA or with DHA plus LA. Investigation of the gelatinolytic activity of the 57-kDa and 92-kDa isoforms of type-IV collagenase (MMP-2 and MMP.9, respectively) showed a clear reduction in the former by treatment with OA, while DHA, but not DHA plus LA or EPA, caused a decrease in the 92-kDa isoform, which was well correlated with AA content in tumor tissues (r = 0.900, p < 0.001). These results suggest that inhibition of metastasis due to treatment with OA and DHA might be due to depressed type-IV collagenase activity.",
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AU - Ishikawa, Chikako

AU - Kuhara, Tetsuya

AU - Asamoto, Makoto

AU - Kunimoto, Takehiko

AU - Moore, Malcolm A.

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AU - Tsuda, Hiroyuki

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