Inhibitory role of the serotonergic system on estrogen receptor α expression in the female rat hypothalamus

Hiroyuki Ito, Yuji Shimogawa, Daisuke Kohagura, Tetsuji Moriizumi, Korehito Yamanouchi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The role of the serotonergic system in regulating the expression of estrogen receptor (ER) α in the hypothalamus was investigated in ovariectomized rats by injecting a serotonin synthesis inhibitor, parachlorophenylalanine (PCPA), or by destroying the dorsal raphe nucleus (DR). The number of ERα-immunoreactive (ir) cells was counted in the anteroventral periventricular nucleus in the preoptic area (AVPV), ventrolateral ventromedial hypothalamic nucleus (vlVMN), and arcuate nucleus (ARCN). Seven days after ovariectomy, 100mg/kg PCPA or saline was injected daily for 4 days. Alternatively, radiofrequency lesioning of the DR (DRL) or sham lesions were made on the same time of ovariectomy. One-day after the last injection of PCPA or 7 days after brain surgery, the brain was fixed for immunostaining of ERα and the number of ERα-ir cell were counted in the nuclei of interest. The mean number of ERα-ir cells/mm3 (density) in the AVPV of the PCPA or DRL groups was statistically higher than that in the saline or sham group. In the vlVMN and ARCN of the PCPA or DRL groups, the mean density of ERα-ir cells was comparable to the saline or sham groups. These results suggest that the serotonergic system of the DR plays an inhibitory role on the expression of ERα in the AVPV, but not in the vlVMN and ARCN.

Original languageEnglish
Pages (from-to)194-198
Number of pages5
JournalNeuroscience Letters
Volume583
DOIs
Publication statusPublished - 2014 Nov 7

Fingerprint

Estrogen Receptors
Hypothalamus
Ventromedial Hypothalamic Nucleus
Arcuate Nucleus of Hypothalamus
Ovariectomy
Anterior Hypothalamus
Preoptic Area
Brain
Serotonin
Cell Count
Injections
Dorsal Raphe Nucleus

Keywords

  • Dorsal raphe nucleus
  • Estrogen receptor α
  • Female rats
  • Inhibition
  • Parachlorophenylalanine
  • Serotonin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Inhibitory role of the serotonergic system on estrogen receptor α expression in the female rat hypothalamus. / Ito, Hiroyuki; Shimogawa, Yuji; Kohagura, Daisuke; Moriizumi, Tetsuji; Yamanouchi, Korehito.

In: Neuroscience Letters, Vol. 583, 07.11.2014, p. 194-198.

Research output: Contribution to journalArticle

Ito, Hiroyuki ; Shimogawa, Yuji ; Kohagura, Daisuke ; Moriizumi, Tetsuji ; Yamanouchi, Korehito. / Inhibitory role of the serotonergic system on estrogen receptor α expression in the female rat hypothalamus. In: Neuroscience Letters. 2014 ; Vol. 583. pp. 194-198.
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