Initiation of functional synapses is associated with AMPA receptor expression

Koji Chono, Hatsuki Shiga, Takuro Tojima, Etsuro Ito

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In the present study, we identified the key protein that modulates the initiation of functional glutamatergic synapses in developing cortical neurons. First, we found a day in vitro that marked a critical increase in the number of functional synapses by the application of cyclic AMP, as demonstrated with Ca2+ imaging. We then examined the changes in the expression levels of proteins, which were expected to play a role in glutamatergic synapses, by the application of cyclic AMP. Our findings suggest that the expression of the α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors modulates the initiation of functional synapses in developing neurons, and that immature neurons already contain N-methyl D-aspartate (NMDA) receptors and presynaptic proteins such as synaptophysin.

Original languageEnglish
Pages (from-to)24-31
Number of pages8
JournalNeuroscience Research Communications
Volume35
Issue number1
DOIs
Publication statusPublished - 2004 Jul
Externally publishedYes

Fingerprint

AMPA Receptors
Synapses
Neurons
Cyclic AMP
Isoxazoles
Synaptophysin
Proteins
N-Methyl-D-Aspartate Receptors

Keywords

  • Ca imaging
  • Cyclic AMP
  • GluR2
  • Glutamate
  • NMDA
  • Oscillation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Initiation of functional synapses is associated with AMPA receptor expression. / Chono, Koji; Shiga, Hatsuki; Tojima, Takuro; Ito, Etsuro.

In: Neuroscience Research Communications, Vol. 35, No. 1, 07.2004, p. 24-31.

Research output: Contribution to journalArticle

Chono, Koji ; Shiga, Hatsuki ; Tojima, Takuro ; Ito, Etsuro. / Initiation of functional synapses is associated with AMPA receptor expression. In: Neuroscience Research Communications. 2004 ; Vol. 35, No. 1. pp. 24-31.
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