Initiation of functional synapses is associated with AMPA receptor expression

Koji Chono, Hatsuki Shiga, Takuro Tojima, Etsuro Ito

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1 Citation (Scopus)


In the present study, we identified the key protein that modulates the initiation of functional glutamatergic synapses in developing cortical neurons. First, we found a day in vitro that marked a critical increase in the number of functional synapses by the application of cyclic AMP, as demonstrated with Ca2+ imaging. We then examined the changes in the expression levels of proteins, which were expected to play a role in glutamatergic synapses, by the application of cyclic AMP. Our findings suggest that the expression of the α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors modulates the initiation of functional synapses in developing neurons, and that immature neurons already contain N-methyl D-aspartate (NMDA) receptors and presynaptic proteins such as synaptophysin.

Original languageEnglish
Pages (from-to)24-31
Number of pages8
JournalNeuroscience Research Communications
Issue number1
Publication statusPublished - 2004 Jul
Externally publishedYes



  • Ca imaging
  • Cyclic AMP
  • GluR2
  • Glutamate
  • NMDA
  • Oscillation

ASJC Scopus subject areas

  • Neuroscience(all)

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