Insulin-like growth factor-I stimulates endothelin-3 secretion from rat anterior pituitary cells in primary culture

Hirokazu Matsumoto, Nobuhiro Suzuki, Kunio Shiota, Kinji Inoue, Masao Tsuda, Masahiko Fujino

Research output: Contribution to journalArticle

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Abstract

Since we found relatively high concentrations of immunoreactive (ir-) ET-3 in the rat pituitary gland (190 pg/g tissue), we have investigated the possible ET-3 secretion from the primary culture of anterior pituitary cells and the effects of various growth factors on the ET-3 secretion. The ir-ET-3 was detected in the incubation medium within 2 h, and 24 h of culture attained the concentrations of 1.15 ± 0.26 pg/well/6 × 105 cells. The ir-ET-3 secretion was stimulated by insulin, insulin like growth factor-II (IGF-II), and most effectively by insulin like growth factor-I (IGF-I) in a dose- and time-dependent manner, whereas the production of ir-ET-1 and ir-big ET-1 was slightly inhibited by IGF-I and IGF-II. In reverse-phase HPLC, the ir-ET-3 released into the culture media showed identical retention time with authentic ET-3. Although ir-ET-1 and ir-big ET-1 secretion was stimulated by transforming growth factor-β (TGF-β), ir-ET-3 secretion was inhibited. These results indicate that the anterior pituitary cells secrete ET-3 and the secretion is stimulated by IGF-I.

Original languageEnglish
Pages (from-to)661-668
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume172
Issue number2
DOIs
Publication statusPublished - 1990 Oct 30
Externally publishedYes

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Endothelin-3
Primary Cell Culture
Insulin-Like Growth Factor I
Rats
Insulin-Like Growth Factor II
Transforming Growth Factors
Pituitary Gland
Culture Media
Intercellular Signaling Peptides and Proteins
High Pressure Liquid Chromatography
Insulin
Tissue

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Insulin-like growth factor-I stimulates endothelin-3 secretion from rat anterior pituitary cells in primary culture. / Matsumoto, Hirokazu; Suzuki, Nobuhiro; Shiota, Kunio; Inoue, Kinji; Tsuda, Masao; Fujino, Masahiko.

In: Biochemical and Biophysical Research Communications, Vol. 172, No. 2, 30.10.1990, p. 661-668.

Research output: Contribution to journalArticle

Matsumoto, Hirokazu ; Suzuki, Nobuhiro ; Shiota, Kunio ; Inoue, Kinji ; Tsuda, Masao ; Fujino, Masahiko. / Insulin-like growth factor-I stimulates endothelin-3 secretion from rat anterior pituitary cells in primary culture. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 172, No. 2. pp. 661-668.
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