Abstract
Since we found relatively high concentrations of immunoreactive (ir-) ET-3 in the rat pituitary gland (190 pg/g tissue), we have investigated the possible ET-3 secretion from the primary culture of anterior pituitary cells and the effects of various growth factors on the ET-3 secretion. The ir-ET-3 was detected in the incubation medium within 2 h, and 24 h of culture attained the concentrations of 1.15 ± 0.26 pg/well/6 × 105 cells. The ir-ET-3 secretion was stimulated by insulin, insulin like growth factor-II (IGF-II), and most effectively by insulin like growth factor-I (IGF-I) in a dose- and time-dependent manner, whereas the production of ir-ET-1 and ir-big ET-1 was slightly inhibited by IGF-I and IGF-II. In reverse-phase HPLC, the ir-ET-3 released into the culture media showed identical retention time with authentic ET-3. Although ir-ET-1 and ir-big ET-1 secretion was stimulated by transforming growth factor-β (TGF-β), ir-ET-3 secretion was inhibited. These results indicate that the anterior pituitary cells secrete ET-3 and the secretion is stimulated by IGF-I.
| Original language | English |
|---|---|
| Pages (from-to) | 661-668 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 172 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1990 Oct 30 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Insulin-like growth factor-I stimulates endothelin-3 secretion from rat anterior pituitary cells in primary culture. / Matsumoto, Hirokazu; Suzuki, Nobuhiro; Shiota, Kunio; Inoue, Kinji; Tsuda, Masao; Fujino, Masahiko.
In: Biochemical and Biophysical Research Communications, Vol. 172, No. 2, 30.10.1990, p. 661-668.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Insulin-like growth factor-I stimulates endothelin-3 secretion from rat anterior pituitary cells in primary culture
AU - Matsumoto, Hirokazu
AU - Suzuki, Nobuhiro
AU - Shiota, Kunio
AU - Inoue, Kinji
AU - Tsuda, Masao
AU - Fujino, Masahiko
PY - 1990/10/30
Y1 - 1990/10/30
N2 - Since we found relatively high concentrations of immunoreactive (ir-) ET-3 in the rat pituitary gland (190 pg/g tissue), we have investigated the possible ET-3 secretion from the primary culture of anterior pituitary cells and the effects of various growth factors on the ET-3 secretion. The ir-ET-3 was detected in the incubation medium within 2 h, and 24 h of culture attained the concentrations of 1.15 ± 0.26 pg/well/6 × 105 cells. The ir-ET-3 secretion was stimulated by insulin, insulin like growth factor-II (IGF-II), and most effectively by insulin like growth factor-I (IGF-I) in a dose- and time-dependent manner, whereas the production of ir-ET-1 and ir-big ET-1 was slightly inhibited by IGF-I and IGF-II. In reverse-phase HPLC, the ir-ET-3 released into the culture media showed identical retention time with authentic ET-3. Although ir-ET-1 and ir-big ET-1 secretion was stimulated by transforming growth factor-β (TGF-β), ir-ET-3 secretion was inhibited. These results indicate that the anterior pituitary cells secrete ET-3 and the secretion is stimulated by IGF-I.
AB - Since we found relatively high concentrations of immunoreactive (ir-) ET-3 in the rat pituitary gland (190 pg/g tissue), we have investigated the possible ET-3 secretion from the primary culture of anterior pituitary cells and the effects of various growth factors on the ET-3 secretion. The ir-ET-3 was detected in the incubation medium within 2 h, and 24 h of culture attained the concentrations of 1.15 ± 0.26 pg/well/6 × 105 cells. The ir-ET-3 secretion was stimulated by insulin, insulin like growth factor-II (IGF-II), and most effectively by insulin like growth factor-I (IGF-I) in a dose- and time-dependent manner, whereas the production of ir-ET-1 and ir-big ET-1 was slightly inhibited by IGF-I and IGF-II. In reverse-phase HPLC, the ir-ET-3 released into the culture media showed identical retention time with authentic ET-3. Although ir-ET-1 and ir-big ET-1 secretion was stimulated by transforming growth factor-β (TGF-β), ir-ET-3 secretion was inhibited. These results indicate that the anterior pituitary cells secrete ET-3 and the secretion is stimulated by IGF-I.
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U2 - 10.1016/0006-291X(90)90725-3
DO - 10.1016/0006-291X(90)90725-3
M3 - Article
VL - 172
SP - 661
EP - 668
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -