Integration in a multilayer microfluidic chip of 8 parallel cell sorters with flow control by sol-gel transition of thermoreversible gelation polymer

Hirokazu Sugino, Takahiro Arakawa, Yuki Nara, Yoshitaka Shirasaki, Kazuto Ozaki, Shuichi Shoji, Takashi Funatsu

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Microfluidic systems have significant implications in the field of cell separation since they could provide platforms with inexpensive, disposable and sterile structures. Here, we present a novel strategy to integrate microfluidic sorters into a single chip for high throughput sorting. Our parallel sorter consists of a microfluidic chip with a three-dimensional channel network that utilizes flow switching by a heat-induced sol-gel transition of thermoreversible gelation polymer. The 8 parallel sheathed sample flows were realized by injecting sample and buffer solutions into only 2 inlets. The sheathed flows enabled disposal of unwanted sample waste without laser irradiation, and collection of wanted sample upon irradiation. As an application of the sorter, two kinds of fluorescent microspheres were separated with recovery ratio and purity of 70% or 90% at throughputs of about 100 or 20 particles per second, respectively. Next, Escherichia coli cells expressing green fluorescent protein were separated from those expressing DsRed with recovery ratio and purity of 90% at a throughput of about 20 cells per second.

    Original languageEnglish
    Pages (from-to)2559-2565
    Number of pages7
    JournalLab on a Chip - Miniaturisation for Chemistry and Biology
    Volume10
    Issue number19
    DOIs
    Publication statusPublished - 2010 Oct 7

    Fingerprint

    Microfluidics
    Polymethyl Methacrylate
    Gelation
    Flow control
    Sol-gels
    Polymers
    Multilayers
    Gels
    Throughput
    Recovery
    Cell Separation
    Laser beam effects
    Green Fluorescent Proteins
    Microspheres
    Sorting
    Waste disposal
    Escherichia coli
    Buffers
    Lasers
    Hot Temperature

    ASJC Scopus subject areas

    • Biochemistry
    • Chemistry(all)
    • Bioengineering
    • Biomedical Engineering

    Cite this

    Integration in a multilayer microfluidic chip of 8 parallel cell sorters with flow control by sol-gel transition of thermoreversible gelation polymer. / Sugino, Hirokazu; Arakawa, Takahiro; Nara, Yuki; Shirasaki, Yoshitaka; Ozaki, Kazuto; Shoji, Shuichi; Funatsu, Takashi.

    In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 10, No. 19, 07.10.2010, p. 2559-2565.

    Research output: Contribution to journalArticle

    Sugino, Hirokazu ; Arakawa, Takahiro ; Nara, Yuki ; Shirasaki, Yoshitaka ; Ozaki, Kazuto ; Shoji, Shuichi ; Funatsu, Takashi. / Integration in a multilayer microfluidic chip of 8 parallel cell sorters with flow control by sol-gel transition of thermoreversible gelation polymer. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2010 ; Vol. 10, No. 19. pp. 2559-2565.
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