Interaction between Ca2+ and dipalmitoylphosphatidylcholine membranes. II. Fluorescence anisotropy study

Ryoichi Kataoka, Shuji Aruga, Shigeki Mitaku, Kazuhiko Kinosita, Akira Ikegami

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The effect of Ca2+ on the molecular mobility in dipalmitoylphosphatidylcholine membranes was studied by steady-state and time-resolved measurements of fluorescence anisotropy. The fluorescence anisotropy decay of 1,6-diphenyl-1,3,5-hexatriene in the hydrocarbon region indicated that the free volume of molecular rotation became more restricted when the Ca2+ concentration was increased. The decrease of the molecular mobility was observed from 1 mM Ca2+, at which the number of bound Ca2+ is much less than that of the total lipid molecules. A distinct difference between Ca2+ and Mg2+ effects suggested that the change in various membrane properties was induced by the binding of these ions. From these results we propose a long-range attractive interaction between bound Ca2+ and the polar head groups of distant phosphatidylcholine molecules.

Original languageEnglish
Pages (from-to)277-284
Number of pages8
JournalBiophysical Chemistry
Volume21
Issue number3-4
DOIs
Publication statusPublished - 1985
Externally publishedYes

Keywords

  • Ca
  • Dipalmitoylphosphatidylcholine
  • Fluorescence anisotropy

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Physical and Theoretical Chemistry

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