Interaction of antiproliferative protein Tob with the CCR4-NOT deadenylase complex

Takashi Miyasaka, Masahiro Morita, Kentaro Ito, Toru Suzuki, Hiroyuki Fukuda, Shizu Takeda, Jun Ichiro Inoue, Kentaro Senba, Tadashi Yamamoto

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    Tob protein, when overexpressed, suppresses growth of NIH3T3 cells, presumably by regulating expression of various growth-related genes. However, the molecular mechanisms underlying Tob-mediated regulation of gene expression have been obscure. To address this issue we established stable Tob-expressing cell lines and used a proteomics approach to identify Tob-interacting proteins. We found that Tob associates with the CCR4-NOT complex. The carboxyl-terminal half of Tob interacted with Cnot1, a core protein of the CCR4-NOT complex. We further showed that the deadenylase activity associated with the complex was suppressed in vitro by Tob. These results suggest that the antiproliferative activity of Tob is shown post-transcriptionally by controlling the stability of the target mRNAs in addition to its involvement in transcriptional regulation, reported previously.

    Original languageEnglish
    Pages (from-to)755-761
    Number of pages7
    JournalCancer Science
    Volume99
    Issue number4
    DOIs
    Publication statusPublished - 2008 Apr

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    Proteins
    RNA Stability
    Gene Expression Regulation
    Growth
    Proteomics
    Cell Line
    Genes
    In Vitro Techniques

    ASJC Scopus subject areas

    • Cancer Research
    • Oncology

    Cite this

    Miyasaka, T., Morita, M., Ito, K., Suzuki, T., Fukuda, H., Takeda, S., ... Yamamoto, T. (2008). Interaction of antiproliferative protein Tob with the CCR4-NOT deadenylase complex. Cancer Science, 99(4), 755-761. https://doi.org/10.1111/j.1349-7006.2008.00746.x

    Interaction of antiproliferative protein Tob with the CCR4-NOT deadenylase complex. / Miyasaka, Takashi; Morita, Masahiro; Ito, Kentaro; Suzuki, Toru; Fukuda, Hiroyuki; Takeda, Shizu; Inoue, Jun Ichiro; Senba, Kentaro; Yamamoto, Tadashi.

    In: Cancer Science, Vol. 99, No. 4, 04.2008, p. 755-761.

    Research output: Contribution to journalArticle

    Miyasaka, T, Morita, M, Ito, K, Suzuki, T, Fukuda, H, Takeda, S, Inoue, JI, Senba, K & Yamamoto, T 2008, 'Interaction of antiproliferative protein Tob with the CCR4-NOT deadenylase complex', Cancer Science, vol. 99, no. 4, pp. 755-761. https://doi.org/10.1111/j.1349-7006.2008.00746.x
    Miyasaka, Takashi ; Morita, Masahiro ; Ito, Kentaro ; Suzuki, Toru ; Fukuda, Hiroyuki ; Takeda, Shizu ; Inoue, Jun Ichiro ; Senba, Kentaro ; Yamamoto, Tadashi. / Interaction of antiproliferative protein Tob with the CCR4-NOT deadenylase complex. In: Cancer Science. 2008 ; Vol. 99, No. 4. pp. 755-761.
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    AU - Takeda, Shizu

    AU - Inoue, Jun Ichiro

    AU - Senba, Kentaro

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