Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cells

Yasuhiko Terada, Yumi Uetake, Ryoko Kuriyama

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

A mitosis-specific Aurora-A kinase has been implicated in microtubule organization and spindle assembly in diverse organisms. However, exactly how Aurora-A controls the microtubule nucleation onto centrosomes is unknown. Here, we show that Aurora-A specifically binds to the COOH-terminal domain of a Drosophila centrosomal protein, centrosomin (CNN), which has been shown to be important for assembly of mitotic spindles and spindle poles. Aurora-A and CNN are mutually dependent for localization at spindle poles, which is required for proper targeting of γ-tubulin and other centrosomal components to the centrosome. The NH2-terminal half of CNN interacts with γ-tubulin, and induces cytoplasmic foci that can initiate microtubule nucleation in vivo and in vitro in both Drosophila and mammalian cells. These results suggest that Aurora-A regulates centrosome assembly by controlling the CNN's ability to targeting and/or anchoring γ-tubulin to the centrosome and organizing microtubule-nucleating sites via its interaction with the COOH-terminal sequence of CNN.

Original languageEnglish
Pages (from-to)757-763
Number of pages7
JournalJournal of Cell Biology
Volume162
Issue number5
DOIs
Publication statusPublished - 2003 Sep 1
Externally publishedYes

Fingerprint

Centrosome
Microtubules
Drosophila
Tubulin
Spindle Poles
Aurora Kinase A
Spindle Apparatus
Mitosis

Keywords

  • γ-tubulin
  • Centrosomes
  • Microtubule nucleation
  • Mitotic spindle
  • MTOC

ASJC Scopus subject areas

  • Cell Biology

Cite this

Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cells. / Terada, Yasuhiko; Uetake, Yumi; Kuriyama, Ryoko.

In: Journal of Cell Biology, Vol. 162, No. 5, 01.09.2003, p. 757-763.

Research output: Contribution to journalArticle

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AB - A mitosis-specific Aurora-A kinase has been implicated in microtubule organization and spindle assembly in diverse organisms. However, exactly how Aurora-A controls the microtubule nucleation onto centrosomes is unknown. Here, we show that Aurora-A specifically binds to the COOH-terminal domain of a Drosophila centrosomal protein, centrosomin (CNN), which has been shown to be important for assembly of mitotic spindles and spindle poles. Aurora-A and CNN are mutually dependent for localization at spindle poles, which is required for proper targeting of γ-tubulin and other centrosomal components to the centrosome. The NH2-terminal half of CNN interacts with γ-tubulin, and induces cytoplasmic foci that can initiate microtubule nucleation in vivo and in vitro in both Drosophila and mammalian cells. These results suggest that Aurora-A regulates centrosome assembly by controlling the CNN's ability to targeting and/or anchoring γ-tubulin to the centrosome and organizing microtubule-nucleating sites via its interaction with the COOH-terminal sequence of CNN.

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