Interaction of Aurora-A and centrosomin at the microtubule-nucleating site in Drosophila and mammalian cells

Yasuhiko Terada, Yumi Uetake, Ryoko Kuriyama

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105 Citations (Scopus)

Abstract

A mitosis-specific Aurora-A kinase has been implicated in microtubule organization and spindle assembly in diverse organisms. However, exactly how Aurora-A controls the microtubule nucleation onto centrosomes is unknown. Here, we show that Aurora-A specifically binds to the COOH-terminal domain of a Drosophila centrosomal protein, centrosomin (CNN), which has been shown to be important for assembly of mitotic spindles and spindle poles. Aurora-A and CNN are mutually dependent for localization at spindle poles, which is required for proper targeting of γ-tubulin and other centrosomal components to the centrosome. The NH2-terminal half of CNN interacts with γ-tubulin, and induces cytoplasmic foci that can initiate microtubule nucleation in vivo and in vitro in both Drosophila and mammalian cells. These results suggest that Aurora-A regulates centrosome assembly by controlling the CNN's ability to targeting and/or anchoring γ-tubulin to the centrosome and organizing microtubule-nucleating sites via its interaction with the COOH-terminal sequence of CNN.

Original languageEnglish
Pages (from-to)757-763
Number of pages7
JournalJournal of Cell Biology
Volume162
Issue number5
DOIs
Publication statusPublished - 2003 Sep 1

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Keywords

  • Centrosomes
  • MTOC
  • Microtubule nucleation
  • Mitotic spindle
  • γ-tubulin

ASJC Scopus subject areas

  • Cell Biology

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