Interaction of hemoglobin vesicles, a cellular-type artificial oxygen carrier, with human plasma: Effects on coagulation, kallikrein-kinin, and complement systems

Hideki Abe, Mitsuhiro Fujihara, Hiroshi Azuma, Hisami Ikeda, Kenji Ikebuchi, Shinji Takeoka, Eishun Tsuchida, Hideyoshi Harashima

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Hemoglobin vesicles (HbVs), cellular-type artificial oxygen carriers containing human hemoglobin, were assessed for their biocompatibility by mixing with human plasma in vitro. Among three kinds of HbVs (PEG-DPEA-HbV, PEG-DPPG-HbV and DPPG-HbV), PEG-DPEA-HbV did not affect the extrinsic or intrinsic coagulation activities of the plasma, while PEG-DPPG-HbV and DPPG-HbV tended to shorten the intrinsic coagulation time. The kallikrein-kinin cascade of the plasma was slightly activated by PEG-DPPG-HbV and DPPG-HbV, but not by PEG-DPEA-HbV. The complement consumption of the plasma was observed by incubation with DPPG-HbV, but not with PEG-DPEA-HbV or PEG-DPPG-HbV. These results indicate that PEG-DPEA-HbV has a higher biocompatibility with human plasma.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalArtificial Cells, Blood Substitutes, and Biotechnology
Volume34
Issue number1
DOIs
Publication statusPublished - 2006 Apr 1

Keywords

  • Complement
  • Hemoglobin vesicles
  • Kallikrein-Kinin
  • Liposome
  • Oxygen carrier

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering

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