Interaction of hemoglobin vesicles, a cellular-type artificial oxygen carrier, with human plasma: Effects on coagulation, kallikrein-kinin, and complement systems

Hideki Abe, Mitsuhiro Fujihara, Hiroshi Azuma, Hisami Ikeda, Kenji Ikebuchi, Shinji Takeoka, Eishun Tsuchida, Hideyoshi Harashima

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    19 Citations (Scopus)

    Abstract

    Hemoglobin vesicles (HbVs), cellular-type artificial oxygen carriers containing human hemoglobin, were assessed for their biocompatibility by mixing with human plasma in vitro. Among three kinds of HbVs (PEG-DPEA-HbV, PEG-DPPG-HbV and DPPG-HbV), PEG-DPEA-HbV did not affect the extrinsic or intrinsic coagulation activities of the plasma, while PEG-DPPG-HbV and DPPG-HbV tended to shorten the intrinsic coagulation time. The kallikrein-kinin cascade of the plasma was slightly activated by PEG-DPPG-HbV and DPPG-HbV, but not by PEG-DPEA-HbV. The complement consumption of the plasma was observed by incubation with DPPG-HbV, but not with PEG-DPEA-HbV or PEG-DPPG-HbV. These results indicate that PEG-DPEA-HbV has a higher biocompatibility with human plasma.

    Original languageEnglish
    Pages (from-to)1-10
    Number of pages10
    JournalArtificial Cells, Blood Substitutes, and Biotechnology
    Volume34
    Issue number1
    DOIs
    Publication statusPublished - 2006 Apr 1

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    Keywords

    • Complement
    • Hemoglobin vesicles
    • Kallikrein-Kinin
    • Liposome
    • Oxygen carrier

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Hematology
    • Biotechnology
    • Biomaterials

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