Interaction of mitochondrial elongation factors Tu.Ts with aminoacyl-tRNA.

L. A. Benkowski, C. Takemoto, G. Ott, M. Beikman, Takuya Ueda, K. Watanabe, M. Sprinzl, L. L. Spremulli

Research output: Contribution to journalArticle

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Abstract

The interaction between the bovine mitochondrial translational elongation factor Tu.Ts complex (EF-Tu.Tsmt) and aminoacyl-tRNA has been investigated using a nuclease protection assay and fluorescence enhancement of [AEDANS-s2C]Tyr-tRNA(Tyr). The equilibrium dissociation constant, Kd, for the EF-Tu.Tsmt:GTP:E. coli Phe-tRNA complex is approximately 50 nM. A similar binding constant (30 nM) is obtained using bovine mitochondrial Phe-tRNA. The equilibrium binding constant for the EF-Tu.Tsmt:GTP:yeast [AEDANS-s2C]Tyr-tRNA(Tyr) complex is approximately 4 nM when determined using the fluorescence enhancement assay.

Original languageEnglish
Pages (from-to)163-166
Number of pages4
JournalNucleic acids symposium series
Issue number33
Publication statusPublished - 1995 Jan 1
Externally publishedYes

Fingerprint

RNA, Transfer, Tyr
RNA, Transfer, Phe
Transfer RNA
Guanosine Triphosphate
Nuclease Protection Assays
Fluorescence
Yeasts
Escherichia coli
elongation factor Ts

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Benkowski, L. A., Takemoto, C., Ott, G., Beikman, M., Ueda, T., Watanabe, K., ... Spremulli, L. L. (1995). Interaction of mitochondrial elongation factors Tu.Ts with aminoacyl-tRNA. Nucleic acids symposium series, (33), 163-166.

Interaction of mitochondrial elongation factors Tu.Ts with aminoacyl-tRNA. / Benkowski, L. A.; Takemoto, C.; Ott, G.; Beikman, M.; Ueda, Takuya; Watanabe, K.; Sprinzl, M.; Spremulli, L. L.

In: Nucleic acids symposium series, No. 33, 01.01.1995, p. 163-166.

Research output: Contribution to journalArticle

Benkowski, LA, Takemoto, C, Ott, G, Beikman, M, Ueda, T, Watanabe, K, Sprinzl, M & Spremulli, LL 1995, 'Interaction of mitochondrial elongation factors Tu.Ts with aminoacyl-tRNA.', Nucleic acids symposium series, no. 33, pp. 163-166.
Benkowski LA, Takemoto C, Ott G, Beikman M, Ueda T, Watanabe K et al. Interaction of mitochondrial elongation factors Tu.Ts with aminoacyl-tRNA. Nucleic acids symposium series. 1995 Jan 1;(33):163-166.
Benkowski, L. A. ; Takemoto, C. ; Ott, G. ; Beikman, M. ; Ueda, Takuya ; Watanabe, K. ; Sprinzl, M. ; Spremulli, L. L. / Interaction of mitochondrial elongation factors Tu.Ts with aminoacyl-tRNA. In: Nucleic acids symposium series. 1995 ; No. 33. pp. 163-166.
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