Interaction of mitochondrial elongation factors Tu.Ts with aminoacyl-tRNA.

L. A. Benkowski, C. Takemoto, G. Ott, M. Beikman, T. Ueda, K. Watanabe, M. Sprinzl, L. L. Spremulli

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Abstract

The interaction between the bovine mitochondrial translational elongation factor Tu.Ts complex (EF-Tu.Tsmt) and aminoacyl-tRNA has been investigated using a nuclease protection assay and fluorescence enhancement of [AEDANS-s2C]Tyr-tRNA(Tyr). The equilibrium dissociation constant, Kd, for the EF-Tu.Tsmt:GTP:E. coli Phe-tRNA complex is approximately 50 nM. A similar binding constant (30 nM) is obtained using bovine mitochondrial Phe-tRNA. The equilibrium binding constant for the EF-Tu.Tsmt:GTP:yeast [AEDANS-s2C]Tyr-tRNA(Tyr) complex is approximately 4 nM when determined using the fluorescence enhancement assay.

Original languageEnglish
Pages (from-to)163-166
Number of pages4
JournalNucleic acids symposium series
Issue number33
Publication statusPublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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    Benkowski, L. A., Takemoto, C., Ott, G., Beikman, M., Ueda, T., Watanabe, K., Sprinzl, M., & Spremulli, L. L. (1995). Interaction of mitochondrial elongation factors Tu.Ts with aminoacyl-tRNA. Nucleic acids symposium series, (33), 163-166.