Interaction of starfish gonadotropin with its receptor: Effect of chimeric relaxin-like gonad-stimulating peptides

Masatoshi Mita, Keitaro Nakamura, Kazuyoshi Tsutsui, Hidekazu Katayama

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    A relaxin-like gonad-stimulating peptide (RGP) of starfish Patiria (Asterina) pectinifera is the first identified invertebrate gonadotropin for final gamete maturation. Recently, we found three orthologs of RGP in the class Asteroida; PpeRGP in P. pectinifera, AamRGP in Asterias amurensis, and AjaRGP in Aphelasterias japonica. In this study, nine kinds of RGP derivatives with exchanged each A- and B-chain were synthesized chemically to analyze the interaction of RGP with its receptor. Among these RGP derivatives, PpeRGP and its chimeric RGPs with B-chains from AamRGP or AjaRGP could induce oocyte maturation and ovulation in P. pectinifera ovaries. In contrast, other RGP derivatives were failed to induce spawning in P. pectinifera ovaries. Circular dichroism spectra of PpeRGP were similar to those of chimeric RGPs with the B-chains from AamRGP or AjaRGP. Furthermore, the predicted three-dimensional structure models of the B-chains from RGP derivatives have almost the same conformation. These findings suggest that the B-chain of PpeRGP is involved in binding to its receptor. Thus, it is likely that the A-chain of AamRGP or AjaRGP disturbs the binding of the PpeRGP B-chain to its receptor.

    Original languageEnglish
    Pages (from-to)30-36
    Number of pages7
    JournalGeneral and Comparative Endocrinology
    Volume276
    DOIs
    Publication statusPublished - 2019 May 15

    Fingerprint

    relaxin
    Starfish
    Relaxin
    Asteroidea
    Gonads
    gonadotropins
    Gonadotropins
    gonads
    peptides
    Peptides
    receptors
    Ovary
    Asterina
    Asterina pectinifera
    Asterias
    circular dichroism spectroscopy
    Invertebrates
    Circular Dichroism
    Ovulation
    Germ Cells

    Keywords

    • Chimeric derivatives
    • Interaction with receptor
    • Relaxin-like gonad-stimulating peptide
    • Species specificity
    • Starfish gonadotropin

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • Endocrinology

    Cite this

    Interaction of starfish gonadotropin with its receptor : Effect of chimeric relaxin-like gonad-stimulating peptides. / Mita, Masatoshi; Nakamura, Keitaro; Tsutsui, Kazuyoshi; Katayama, Hidekazu.

    In: General and Comparative Endocrinology, Vol. 276, 15.05.2019, p. 30-36.

    Research output: Contribution to journalArticle

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