Interaction of Tomato mosaic virus movement protein with tobacco RIO kinase

Kuniaki Yoshioka, Yasuhiko Matsushita, Masahiro Kasahara, Ken Ichi Konagaya, Hiroshi Nyunoya

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Tomato mosaic virus (ToMV) has a regulatory gene encoding a movement protein (MP) that is involved in the cell-to-cell movement of viral RNA through plasmodesmata. To identify the host cell factors interacting with ToMV MP, we used a recombinant MP probe to isolate cDNA clones from a phage expression library of Nicotiana tabacum by a far-Western screening method. One of the cDNA clones encoded an MP-interacting protein, MIP-T7, homologous to the yeast novel protein kinase, Rio1p. We isolated a full-length cDNA by RT-PCR. The putative gene product was designated NtRIO, and shared 33 and 73% amino acid identity with yeast and Arabidopsis RIO kinases, respectively. In vitro analyses using recombinant proteins showed that NtRIO also interacted with a different MP derived from Cucumber mosaic virus. NtRIO had autophosphorylation activity and phosphorylated ToMV MP. Addition of recombinant tobacco casein kinase 2 resulted in a marked increase in the phosphorylation of NtRIO. The interaction between NtRIO and ToMV MP was inhibited by phosphorylation of NtRIO.

Original languageEnglish
Pages (from-to)223-229
Number of pages7
JournalMolecules and Cells
Volume17
Issue number2
Publication statusPublished - 2004 Apr 30
Externally publishedYes

Fingerprint

Mosaic Viruses
Lycopersicon esculentum
Tobacco
Phosphotransferases
Proteins
Complementary DNA
Recombinant Proteins
Clone Cells
Plasmodesmata
Phosphorylation
Cucumovirus
Casein Kinase II
Fungal Proteins
Viral RNA
Regulator Genes
Arabidopsis
Bacteriophages
Protein Kinases
Cell Movement
Yeasts

Keywords

  • Casein kinase 2
  • Far-western
  • Movement protein
  • Nicotiana tabacum
  • Plant virus
  • Protein interaction
  • Protein phosphorylation
  • RIO
  • TMV
  • Tomato mosaic virus

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Yoshioka, K., Matsushita, Y., Kasahara, M., Konagaya, K. I., & Nyunoya, H. (2004). Interaction of Tomato mosaic virus movement protein with tobacco RIO kinase. Molecules and Cells, 17(2), 223-229.

Interaction of Tomato mosaic virus movement protein with tobacco RIO kinase. / Yoshioka, Kuniaki; Matsushita, Yasuhiko; Kasahara, Masahiro; Konagaya, Ken Ichi; Nyunoya, Hiroshi.

In: Molecules and Cells, Vol. 17, No. 2, 30.04.2004, p. 223-229.

Research output: Contribution to journalArticle

Yoshioka, K, Matsushita, Y, Kasahara, M, Konagaya, KI & Nyunoya, H 2004, 'Interaction of Tomato mosaic virus movement protein with tobacco RIO kinase', Molecules and Cells, vol. 17, no. 2, pp. 223-229.
Yoshioka K, Matsushita Y, Kasahara M, Konagaya KI, Nyunoya H. Interaction of Tomato mosaic virus movement protein with tobacco RIO kinase. Molecules and Cells. 2004 Apr 30;17(2):223-229.
Yoshioka, Kuniaki ; Matsushita, Yasuhiko ; Kasahara, Masahiro ; Konagaya, Ken Ichi ; Nyunoya, Hiroshi. / Interaction of Tomato mosaic virus movement protein with tobacco RIO kinase. In: Molecules and Cells. 2004 ; Vol. 17, No. 2. pp. 223-229.
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