Intercellular Ca 2+ response triggered by IP 3 among endothelial cells in shear stress flow

Susumu Kudo, Makoto Hosobuchi, Shogo Kuretoko, Tateki Sumii, Tomoya Shimada, Marie Terada, Kazuo Tanishita

Research output: Contribution to journalArticle

Abstract

A phenomenon has been observed in which intracellular Ca 2+ concentration in endothelial cells increases upon application of shear stress (Ca 2+ response). It is therefore assumed that Ca 2+ is the second messenger in the transfer of shear stress stimulation into cells. The Ca 2+ response is also known to spread to surrounding cells (Ca 2+ wave). We investigated the effects on Ca 2+ wave among cultured bovine aorta endothelial cells (BAECs) upon inhibiting the main intercellular signaling pathways, such as gap junction and paracrine pathways by inducing Ca 2+ wave using D-myo-inositol 1,4,5-trisphosphate, P4(5)-(l-(2-nitrophenyl)ethyl) ester trisodium salt (Caged IP 3) due to an intracellular IP 3 elevation. In addition, we investigated the Ca 2+ wave among BAECs under shear stress loading. Using Caged IP 3, local release of ATP from BAEC induced Ca 2+ wave. The Ca 2+ wave was inhibited by the inhibitors of paracrine pathways. Furthermore, the Ca 2+ response spread in the direction of the downstream under shear flow. These results suggest that paracrine pathway is dominant in both of flow and no flow conditions.

Original languageEnglish
Pages (from-to)2431-2441
Number of pages11
JournalNihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B
Volume77
Issue number784
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Endothelial cells
shear stress
Shear stress
aorta
cells
inositols
adenosine triphosphate
Adenosinetriphosphate
Shear flow
stimulation
shear flow
inhibitors
esters
Esters
Salts
salts

Keywords

  • ATP
  • Ca response
  • Endothelial cell
  • IP
  • Paracrine
  • Shear stress

ASJC Scopus subject areas

  • Mechanical Engineering
  • Condensed Matter Physics

Cite this

Intercellular Ca 2+ response triggered by IP 3 among endothelial cells in shear stress flow. / Kudo, Susumu; Hosobuchi, Makoto; Kuretoko, Shogo; Sumii, Tateki; Shimada, Tomoya; Terada, Marie; Tanishita, Kazuo.

In: Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B, Vol. 77, No. 784, 2011, p. 2431-2441.

Research output: Contribution to journalArticle

Kudo, Susumu ; Hosobuchi, Makoto ; Kuretoko, Shogo ; Sumii, Tateki ; Shimada, Tomoya ; Terada, Marie ; Tanishita, Kazuo. / Intercellular Ca 2+ response triggered by IP 3 among endothelial cells in shear stress flow. In: Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B. 2011 ; Vol. 77, No. 784. pp. 2431-2441.
@article{3a72daf1302c457b95812727b28e8c63,
title = "Intercellular Ca 2+ response triggered by IP 3 among endothelial cells in shear stress flow",
abstract = "A phenomenon has been observed in which intracellular Ca 2+ concentration in endothelial cells increases upon application of shear stress (Ca 2+ response). It is therefore assumed that Ca 2+ is the second messenger in the transfer of shear stress stimulation into cells. The Ca 2+ response is also known to spread to surrounding cells (Ca 2+ wave). We investigated the effects on Ca 2+ wave among cultured bovine aorta endothelial cells (BAECs) upon inhibiting the main intercellular signaling pathways, such as gap junction and paracrine pathways by inducing Ca 2+ wave using D-myo-inositol 1,4,5-trisphosphate, P4(5)-(l-(2-nitrophenyl)ethyl) ester trisodium salt (Caged IP 3) due to an intracellular IP 3 elevation. In addition, we investigated the Ca 2+ wave among BAECs under shear stress loading. Using Caged IP 3, local release of ATP from BAEC induced Ca 2+ wave. The Ca 2+ wave was inhibited by the inhibitors of paracrine pathways. Furthermore, the Ca 2+ response spread in the direction of the downstream under shear flow. These results suggest that paracrine pathway is dominant in both of flow and no flow conditions.",
keywords = "ATP, Ca response, Endothelial cell, IP, Paracrine, Shear stress",
author = "Susumu Kudo and Makoto Hosobuchi and Shogo Kuretoko and Tateki Sumii and Tomoya Shimada and Marie Terada and Kazuo Tanishita",
year = "2011",
doi = "10.1299/kikaib.77.2431",
language = "English",
volume = "77",
pages = "2431--2441",
journal = "Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B",
issn = "0387-5016",
publisher = "Japan Society of Mechanical Engineers",
number = "784",

}

TY - JOUR

T1 - Intercellular Ca 2+ response triggered by IP 3 among endothelial cells in shear stress flow

AU - Kudo, Susumu

AU - Hosobuchi, Makoto

AU - Kuretoko, Shogo

AU - Sumii, Tateki

AU - Shimada, Tomoya

AU - Terada, Marie

AU - Tanishita, Kazuo

PY - 2011

Y1 - 2011

N2 - A phenomenon has been observed in which intracellular Ca 2+ concentration in endothelial cells increases upon application of shear stress (Ca 2+ response). It is therefore assumed that Ca 2+ is the second messenger in the transfer of shear stress stimulation into cells. The Ca 2+ response is also known to spread to surrounding cells (Ca 2+ wave). We investigated the effects on Ca 2+ wave among cultured bovine aorta endothelial cells (BAECs) upon inhibiting the main intercellular signaling pathways, such as gap junction and paracrine pathways by inducing Ca 2+ wave using D-myo-inositol 1,4,5-trisphosphate, P4(5)-(l-(2-nitrophenyl)ethyl) ester trisodium salt (Caged IP 3) due to an intracellular IP 3 elevation. In addition, we investigated the Ca 2+ wave among BAECs under shear stress loading. Using Caged IP 3, local release of ATP from BAEC induced Ca 2+ wave. The Ca 2+ wave was inhibited by the inhibitors of paracrine pathways. Furthermore, the Ca 2+ response spread in the direction of the downstream under shear flow. These results suggest that paracrine pathway is dominant in both of flow and no flow conditions.

AB - A phenomenon has been observed in which intracellular Ca 2+ concentration in endothelial cells increases upon application of shear stress (Ca 2+ response). It is therefore assumed that Ca 2+ is the second messenger in the transfer of shear stress stimulation into cells. The Ca 2+ response is also known to spread to surrounding cells (Ca 2+ wave). We investigated the effects on Ca 2+ wave among cultured bovine aorta endothelial cells (BAECs) upon inhibiting the main intercellular signaling pathways, such as gap junction and paracrine pathways by inducing Ca 2+ wave using D-myo-inositol 1,4,5-trisphosphate, P4(5)-(l-(2-nitrophenyl)ethyl) ester trisodium salt (Caged IP 3) due to an intracellular IP 3 elevation. In addition, we investigated the Ca 2+ wave among BAECs under shear stress loading. Using Caged IP 3, local release of ATP from BAEC induced Ca 2+ wave. The Ca 2+ wave was inhibited by the inhibitors of paracrine pathways. Furthermore, the Ca 2+ response spread in the direction of the downstream under shear flow. These results suggest that paracrine pathway is dominant in both of flow and no flow conditions.

KW - ATP

KW - Ca response

KW - Endothelial cell

KW - IP

KW - Paracrine

KW - Shear stress

UR - http://www.scopus.com/inward/record.url?scp=84859612105&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859612105&partnerID=8YFLogxK

U2 - 10.1299/kikaib.77.2431

DO - 10.1299/kikaib.77.2431

M3 - Article

VL - 77

SP - 2431

EP - 2441

JO - Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B

JF - Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B

SN - 0387-5016

IS - 784

ER -