Interdependency of Fission Yeast Alp14/TOG and Coiled Coil Protein Alp7 in Microtubule Localization and Bipolar Spindle Formation

Masamitsu Sato, Leah Vardy, Miguel Angel Garcia, Nirada Koonrugsa, Takashi Toda

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The Dis1/TOG family plays a pivotal role in microtubule organization. In fission yeast, Alp14 and Dis1 share an essential function in bipolar spindle formation. Here, we characterize Alp7, a novel coiled-coil protein that is required for organization of bipolar spindles. Both Alp7 and Alp14 colocalize to the spindle pole body (SPB) and mitotic spindles. Alp14 localization to these sites is fully dependent upon Alp7. Conversely, in the absence of Alp14, Alp7 localizes to the SPBs, but not mitotic spindles. Alp7 forms a complex with Alp14, where the C-terminal region of Alp14 interacts with the coiled-coil domain of Alp7. Intriguingly, this Alp14 C terminus is necessary and sufficient for mitotic spindle localization. Overproduction of either full-length or coiled-coil region of Alp7 results in abnormal V-shaped spindles and stabilization of interphase microtubules, which is induced independent of Alp14. Alp7 may be a functional homologue of animal TACC. Our results shed light on an interdependent relationship between Alp14/TOG and Alp7. We propose a two-step model that accounts for the recruitment of Alp7 and Alp14 to the SPB and microtubules.

Original languageEnglish
Pages (from-to)1609-1622
Number of pages14
JournalMolecular Biology of the Cell
Volume15
Issue number4
DOIs
Publication statusPublished - 2004 Apr
Externally publishedYes

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Spindle Apparatus
Schizosaccharomyces
Spindle Pole Bodies
Microtubules
Proteins
Interphase

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Interdependency of Fission Yeast Alp14/TOG and Coiled Coil Protein Alp7 in Microtubule Localization and Bipolar Spindle Formation. / Sato, Masamitsu; Vardy, Leah; Garcia, Miguel Angel; Koonrugsa, Nirada; Toda, Takashi.

In: Molecular Biology of the Cell, Vol. 15, No. 4, 04.2004, p. 1609-1622.

Research output: Contribution to journalArticle

Sato, Masamitsu ; Vardy, Leah ; Garcia, Miguel Angel ; Koonrugsa, Nirada ; Toda, Takashi. / Interdependency of Fission Yeast Alp14/TOG and Coiled Coil Protein Alp7 in Microtubule Localization and Bipolar Spindle Formation. In: Molecular Biology of the Cell. 2004 ; Vol. 15, No. 4. pp. 1609-1622.
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