Interference with cell-to-cell movement of Tomato mosaic virus by transient overexpression of Arabidopsis thaliana KELP homologs from different plant species

Nobumitsu Sasaki, Tatsuro Odawara, Masakazu Deguchi, Yasuhiko Matsushita, Hiroshi Nyunoya

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The KELP protein of Arabidopsis thaliana (AtKELP), which binds to the Tomato mosaic virus (ToMV) movement protein in vitro, can interfere with cell-to-cell movement of the virus under transient overexpression conditions. In this study, we constructed expression plasmids encoding a fluorescent protein fused to AtKELP or to its homolog from Brassica, Nicotiana, Solanum, or Oryza species. When expressed transiently, all the AtKELP homologs inhibited the cell-to-cell movement of ToMV with different efficiencies. The results of domain swapping between two selected AtKELP homologs suggest the importance of combination between the N- and C-terminal half regions for high inhibitory function.

Original languageEnglish
Pages (from-to)69-73
Number of pages5
JournalJournal of General Plant Pathology
Volume76
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1
Externally publishedYes

Keywords

  • Cell-to-cell movement
  • Inhibitory factor
  • KELP
  • Movement protein
  • Tomato mosaic virus
  • Transient overexpression

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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