Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris

Nobumitsu Sasaki, Tatsuro Odawara, Shoko Nagai, Kazuma Yoshimura, Yasuhiko Matsushita, Hiroshi Nyunoya

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A putative transcriptional coactivator KELP of . Arabidopsis thaliana and its homolog of . Brassica campestris (BcKELP) can bind the movement protein (MP) of . Tomato mosaic virus (ToMV) . in vitro and, when transiently over-expressed, interfere with the cell-to-cell movement of ToMV. In this study, we generated and selected transgenic . Nicotiana tabacum lines expressing BcKELP constitutively and examined their resistance to ToMV. We also investigated changes in MP localization in initially infected . Nicotiana benthamiana cells expressing BcKELP transiently. Our results indicate that a high expression of BcKELP can reduce viral initial and short-distance movement probably through disturbance of plasmodesmal targeting of MP.

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalPhysiological and Molecular Plant Pathology
Volume78
DOIs
Publication statusPublished - 2012 Apr
Externally publishedYes

Fingerprint

Brassica rapa subsp. oleifera
Tomato mosaic virus
Mosaic Viruses
Genetically Modified Plants
Brassica
Lycopersicon esculentum
crossover interference
cell movement
Tobacco
Cell Movement
tobacco
genetically modified organisms
Viruses
viruses
Proteins
proteins
Nicotiana benthamiana
Protein Transport
cells
Arabidopsis

Keywords

  • Cell-to-cell movement
  • Movement protein
  • Plant protection
  • Plasmodesmal targeting
  • Tomato mosaic virus
  • Transcriptional coactivator
  • Transgenic tobacco

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Cite this

@article{d0a0cc6cd5a845368c40fd525dfbae4e,
title = "Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris",
abstract = "A putative transcriptional coactivator KELP of . Arabidopsis thaliana and its homolog of . Brassica campestris (BcKELP) can bind the movement protein (MP) of . Tomato mosaic virus (ToMV) . in vitro and, when transiently over-expressed, interfere with the cell-to-cell movement of ToMV. In this study, we generated and selected transgenic . Nicotiana tabacum lines expressing BcKELP constitutively and examined their resistance to ToMV. We also investigated changes in MP localization in initially infected . Nicotiana benthamiana cells expressing BcKELP transiently. Our results indicate that a high expression of BcKELP can reduce viral initial and short-distance movement probably through disturbance of plasmodesmal targeting of MP.",
keywords = "Cell-to-cell movement, Movement protein, Plant protection, Plasmodesmal targeting, Tomato mosaic virus, Transcriptional coactivator, Transgenic tobacco",
author = "Nobumitsu Sasaki and Tatsuro Odawara and Shoko Nagai and Kazuma Yoshimura and Yasuhiko Matsushita and Hiroshi Nyunoya",
year = "2012",
month = "4",
doi = "10.1016/j.pmpp.2012.01.003",
language = "English",
volume = "78",
pages = "38--44",
journal = "Physiological and Molecular Plant Pathology",
issn = "0885-5765",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris

AU - Sasaki, Nobumitsu

AU - Odawara, Tatsuro

AU - Nagai, Shoko

AU - Yoshimura, Kazuma

AU - Matsushita, Yasuhiko

AU - Nyunoya, Hiroshi

PY - 2012/4

Y1 - 2012/4

N2 - A putative transcriptional coactivator KELP of . Arabidopsis thaliana and its homolog of . Brassica campestris (BcKELP) can bind the movement protein (MP) of . Tomato mosaic virus (ToMV) . in vitro and, when transiently over-expressed, interfere with the cell-to-cell movement of ToMV. In this study, we generated and selected transgenic . Nicotiana tabacum lines expressing BcKELP constitutively and examined their resistance to ToMV. We also investigated changes in MP localization in initially infected . Nicotiana benthamiana cells expressing BcKELP transiently. Our results indicate that a high expression of BcKELP can reduce viral initial and short-distance movement probably through disturbance of plasmodesmal targeting of MP.

AB - A putative transcriptional coactivator KELP of . Arabidopsis thaliana and its homolog of . Brassica campestris (BcKELP) can bind the movement protein (MP) of . Tomato mosaic virus (ToMV) . in vitro and, when transiently over-expressed, interfere with the cell-to-cell movement of ToMV. In this study, we generated and selected transgenic . Nicotiana tabacum lines expressing BcKELP constitutively and examined their resistance to ToMV. We also investigated changes in MP localization in initially infected . Nicotiana benthamiana cells expressing BcKELP transiently. Our results indicate that a high expression of BcKELP can reduce viral initial and short-distance movement probably through disturbance of plasmodesmal targeting of MP.

KW - Cell-to-cell movement

KW - Movement protein

KW - Plant protection

KW - Plasmodesmal targeting

KW - Tomato mosaic virus

KW - Transcriptional coactivator

KW - Transgenic tobacco

UR - http://www.scopus.com/inward/record.url?scp=84856791950&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856791950&partnerID=8YFLogxK

U2 - 10.1016/j.pmpp.2012.01.003

DO - 10.1016/j.pmpp.2012.01.003

M3 - Article

VL - 78

SP - 38

EP - 44

JO - Physiological and Molecular Plant Pathology

JF - Physiological and Molecular Plant Pathology

SN - 0885-5765

ER -