Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris

Nobumitsu Sasaki, Tatsuro Odawara, Shoko Nagai, Kazuma Yoshimura, Yasuhiko Matsushita, Hiroshi Nyunoya

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A putative transcriptional coactivator KELP of . Arabidopsis thaliana and its homolog of . Brassica campestris (BcKELP) can bind the movement protein (MP) of . Tomato mosaic virus (ToMV) . in vitro and, when transiently over-expressed, interfere with the cell-to-cell movement of ToMV. In this study, we generated and selected transgenic . Nicotiana tabacum lines expressing BcKELP constitutively and examined their resistance to ToMV. We also investigated changes in MP localization in initially infected . Nicotiana benthamiana cells expressing BcKELP transiently. Our results indicate that a high expression of BcKELP can reduce viral initial and short-distance movement probably through disturbance of plasmodesmal targeting of MP.

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalPhysiological and Molecular Plant Pathology
Volume78
DOIs
Publication statusPublished - 2012 Apr 1

Keywords

  • Cell-to-cell movement
  • Movement protein
  • Plant protection
  • Plasmodesmal targeting
  • Tomato mosaic virus
  • Transcriptional coactivator
  • Transgenic tobacco

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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