Intergeneric hybridization between aspergillus niger and trichoderma viride by protoplast fusion

Kotaro Kirimura, Masatoshi Imura, Sung Pyo Lee, Yutaka Kato, Shoji Usami, Shoji Usami

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    The intergeneric hybridization between Aspergillus niger and Trichoderma viride was studied. The fusion of protoplasts from auxotrophic mutant strains, using a polyethylene glycol solution, resulted in the formation of intergeneric fusants, the fusion frequencies being 105 to 10 “4. The fusant strains were classifiable into two types according to their morphologies. The fusant strains of the first type formed prototrophic conidia and colonies showing an A. niger type morphology on both the minimal and supplemented media. These fusant strains were proved to be haploids by measurement of conidial sizes and DNA contents, and assumed to be recombinants. The fusant strains of the second type formed conidia showing the same nutritional requirements as those of the original auxotrophic mutant strains used for protoplast fusion. These fusant strains formed colonies showing mixed morphologies, between those of A. niger and T. viride, on the minimal medium and grew more slowly than the prototrophic parental strains of A. niger and T. viride. From these results and those of conidia analyses, the fusant strains of the second type were proved to be heterokaryons. Mycelial protoplasts of the fusant strains of the two types regenerated on the hypertonic minimal medium into colonies with morphological features identical to those of the original fusant strains.

    Original languageEnglish
    Pages (from-to)1589-1596
    Number of pages8
    JournalAgricultural and Biological Chemistry
    Volume53
    Issue number6
    DOIs
    Publication statusPublished - 1989

    Fingerprint

    intergeneric hybridization
    protoplast fusion
    Trichoderma
    Trichoderma viride
    Aspergillus niger
    Protoplasts
    Aspergillus
    Fungal Spores
    Fusion reactions
    Nutritional Requirements
    Haploidy
    conidia
    auxotrophs
    DNA
    heterokaryon
    polyethylene glycol
    nutrient requirements
    haploidy
    protoplasts

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Intergeneric hybridization between aspergillus niger and trichoderma viride by protoplast fusion. / Kirimura, Kotaro; Imura, Masatoshi; Lee, Sung Pyo; Kato, Yutaka; Usami, Shoji; Usami, Shoji.

    In: Agricultural and Biological Chemistry, Vol. 53, No. 6, 1989, p. 1589-1596.

    Research output: Contribution to journalArticle

    Kirimura, Kotaro ; Imura, Masatoshi ; Lee, Sung Pyo ; Kato, Yutaka ; Usami, Shoji ; Usami, Shoji. / Intergeneric hybridization between aspergillus niger and trichoderma viride by protoplast fusion. In: Agricultural and Biological Chemistry. 1989 ; Vol. 53, No. 6. pp. 1589-1596.
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