Interleukin-1-inhibitory IgG in sera from some patients with rheumatoid arthritis

H. Suzuki, Takao Akama, M. Okane, I. Kono, Y. Matsui, K. Yamane, H. Kashiwagi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Inhibition of interleukin-1α (IL-1α) activity was detected in 7 of 41 serum samples from patients with rheumatoid arthritis (RA). These 7 sera inhibited not only IL-1α-induced endothelial cell adherence to neutrophils, but also IL-1β-induced endothelial cell adherence, although to a lesser extent. These sera showed no influence on tumor necrosis factor-induced endothelial cell adherence. No inhibitory activity was found in 40 sera from normal control subjects. Studies to further examine these effects included gel filtration analysis of 2 of the RA sera. The inhibitory activity was eluted near M(r) 158 kd and above M(r) 250 kd. Analysis by protein A affinity chromatography showed that IL-1-inhibitory activity was present in protein A-binding fractions. Purified IgG (by DE-52 column chromatography) from RA patients was found to be as potent an inhibitor as the protein A-binding fractions, which suggests that the major inhibitory activity in RA sera is attributable to IgG molecules. These purified IgG molecules also inhibited IL-1-induced proliferation of mouse thymocytes but did not influence IL-2-dependent proliferation of the CTLL-2 murine T cell line. The 7 patients whose sera showed IL-1-inhibitory activity had mild RA and low titers of rheumatoid factor. The findings, taken together, suggest a possible regulatory role of IL-1-inhibitory IgG in RA disease activity.

Original languageEnglish
Pages (from-to)1528-1538
Number of pages11
JournalArthritis and Rheumatism
Volume32
Issue number12
DOIs
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Interleukin-1
Rheumatoid Arthritis
Immunoglobulin G
Serum
Staphylococcal Protein A
Endothelial Cells
Protein Binding
Rheumatoid Factor
Thymocytes
Affinity Chromatography
Interleukin-2
Gel Chromatography
Chromatography
Neutrophils
Tumor Necrosis Factor-alpha
T-Lymphocytes
Cell Line

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Suzuki, H., Akama, T., Okane, M., Kono, I., Matsui, Y., Yamane, K., & Kashiwagi, H. (1989). Interleukin-1-inhibitory IgG in sera from some patients with rheumatoid arthritis. Arthritis and Rheumatism, 32(12), 1528-1538. https://doi.org/10.1002/anr.1780321206

Interleukin-1-inhibitory IgG in sera from some patients with rheumatoid arthritis. / Suzuki, H.; Akama, Takao; Okane, M.; Kono, I.; Matsui, Y.; Yamane, K.; Kashiwagi, H.

In: Arthritis and Rheumatism, Vol. 32, No. 12, 1989, p. 1528-1538.

Research output: Contribution to journalArticle

Suzuki, H, Akama, T, Okane, M, Kono, I, Matsui, Y, Yamane, K & Kashiwagi, H 1989, 'Interleukin-1-inhibitory IgG in sera from some patients with rheumatoid arthritis', Arthritis and Rheumatism, vol. 32, no. 12, pp. 1528-1538. https://doi.org/10.1002/anr.1780321206
Suzuki, H. ; Akama, Takao ; Okane, M. ; Kono, I. ; Matsui, Y. ; Yamane, K. ; Kashiwagi, H. / Interleukin-1-inhibitory IgG in sera from some patients with rheumatoid arthritis. In: Arthritis and Rheumatism. 1989 ; Vol. 32, No. 12. pp. 1528-1538.
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