Internal motion of F-actin in 10-6-10-3s time range studied by transient absorption anisotropy

Detection of torsional motion

Koshin Mihashi, Hideyuki Yoshimura, Takuhiro Nishio, Akira Ikegami, Kazuhiko Kinosita

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

By means of laser flash photolysis, the transient absorption anisotropy (TAA) of the triplet probe, 5-iodoacetamide-Eosin, labeling rabbit skeletal F-actin was measured in the 10-6-10-3 s time range. The TAA curve at 20°C showed a relatively slow decay phase covering several hundred microseconds and a large residual anisotropy (∼0.1 at 2 ms). After analysis with Barkley & Zimm's formula, it was concluded that the TAA of Eosin-F-actin can be approximated by the anisotropy decay due to torsional motion of F-actin.

Original languageEnglish
Pages (from-to)1705-1707
Number of pages3
JournalJournal of Biochemistry
Volume93
Issue number6
Publication statusPublished - 1983
Externally publishedYes

Fingerprint

Actin
Anisotropy
Actins
Absorption
Internal
Motion
Range of data
Decay
Photolysis
Flash
Rabbit
Eosine Yellowish-(YS)
Labeling
Lasers
Probe
Covering
Laser
Rabbits
Curve

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Internal motion of F-actin in 10-6-10-3s time range studied by transient absorption anisotropy : Detection of torsional motion. / Mihashi, Koshin; Yoshimura, Hideyuki; Nishio, Takuhiro; Ikegami, Akira; Kinosita, Kazuhiko.

In: Journal of Biochemistry, Vol. 93, No. 6, 1983, p. 1705-1707.

Research output: Contribution to journalArticle

Mihashi, K, Yoshimura, H, Nishio, T, Ikegami, A & Kinosita, K 1983, 'Internal motion of F-actin in 10-6-10-3s time range studied by transient absorption anisotropy: Detection of torsional motion', Journal of Biochemistry, vol. 93, no. 6, pp. 1705-1707.
Mihashi, Koshin ; Yoshimura, Hideyuki ; Nishio, Takuhiro ; Ikegami, Akira ; Kinosita, Kazuhiko. / Internal motion of F-actin in 10-6-10-3s time range studied by transient absorption anisotropy : Detection of torsional motion. In: Journal of Biochemistry. 1983 ; Vol. 93, No. 6. pp. 1705-1707.
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AU - Ikegami, Akira

AU - Kinosita, Kazuhiko

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