Internal motion of F-actin in 10-6-10-3s time range studied by transient absorption anisotropy: Detection of torsional motion

Koshin Mihashi, Hideyuki Yoshimura, Takuhiro Nishio, Akira Ikegami, Kazuhiko Kinosita

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Abstract

By means of laser flash photolysis, the transient absorption anisotropy (TAA) of the triplet probe, 5-iodoacetamide-Eosin, labeling rabbit skeletal F-actin was measured in the 10-6-10-3 s time range. The TAA curve at 20°C showed a relatively slow decay phase covering several hundred microseconds and a large residual anisotropy (∼0.1 at 2 ms). After analysis with Barkley & Zimm's formula, it was concluded that the TAA of Eosin-F-actin can be approximated by the anisotropy decay due to torsional motion of F-actin.

Original languageEnglish
Pages (from-to)1705-1707
Number of pages3
JournalJournal of Biochemistry
Volume93
Issue number6
Publication statusPublished - 1983
Externally publishedYes

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ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

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