Interplay between folding/unfolding and helix/coil transitions in giant DNA

Sergey Mikhailenko, Vladimir G. Sergeyev, Anatoly A. Zinchenko, Marat O. Gallyamov, Igor V. Yaminsky, Kenichi Yoshikawa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

It has been well established that double-stranded DNA undergoes a melting, or helix/coil, transition into a single-stranded coil state with an increase in temperature. On the other hand, it has recently been found that, at a fixed temperature, long DNA, larger than several kilobase pairs, exhibits a discrete transition, or switching, between elongated and folded states, preserving its double-stranded structure, with the addition of various condensation agents, such as alcohol, hydrophilic polymer, multivalent cation, and cationic surfactant. In the present study, we examined the interplay between the folding/unfolding transition and the helix/coil transition in individual giant DNA molecules, by observing the conformation of single molecular chains with fluorescence microscopy. The results indicate that the helix-to-coil transition tightly cooperates with the unfolding transition in DNA.

Original languageEnglish
Pages (from-to)597-603
Number of pages7
JournalBiomacromolecules
Volume1
Issue number4
Publication statusPublished - 2000 Dec
Externally publishedYes

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DNA
Molecular Conformation
Temperature
Cationic surfactants
Fluorescence microscopy
Fluorescence Microscopy
Surface-Active Agents
Freezing
Conformations
Cations
Condensation
Polymers
Melting
Alcohols
Positive ions
Molecules

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics

Cite this

Mikhailenko, S., Sergeyev, V. G., Zinchenko, A. A., Gallyamov, M. O., Yaminsky, I. V., & Yoshikawa, K. (2000). Interplay between folding/unfolding and helix/coil transitions in giant DNA. Biomacromolecules, 1(4), 597-603.

Interplay between folding/unfolding and helix/coil transitions in giant DNA. / Mikhailenko, Sergey; Sergeyev, Vladimir G.; Zinchenko, Anatoly A.; Gallyamov, Marat O.; Yaminsky, Igor V.; Yoshikawa, Kenichi.

In: Biomacromolecules, Vol. 1, No. 4, 12.2000, p. 597-603.

Research output: Contribution to journalArticle

Mikhailenko, S, Sergeyev, VG, Zinchenko, AA, Gallyamov, MO, Yaminsky, IV & Yoshikawa, K 2000, 'Interplay between folding/unfolding and helix/coil transitions in giant DNA', Biomacromolecules, vol. 1, no. 4, pp. 597-603.
Mikhailenko S, Sergeyev VG, Zinchenko AA, Gallyamov MO, Yaminsky IV, Yoshikawa K. Interplay between folding/unfolding and helix/coil transitions in giant DNA. Biomacromolecules. 2000 Dec;1(4):597-603.
Mikhailenko, Sergey ; Sergeyev, Vladimir G. ; Zinchenko, Anatoly A. ; Gallyamov, Marat O. ; Yaminsky, Igor V. ; Yoshikawa, Kenichi. / Interplay between folding/unfolding and helix/coil transitions in giant DNA. In: Biomacromolecules. 2000 ; Vol. 1, No. 4. pp. 597-603.
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