Intestinal Dysbiosis and Biotin Deprivation Induce Alopecia through Overgrowth of Lactobacillus murinus in Mice

Atsushi Hayashi, Yohei Mikami, Kentaro Miyamoto, Nobuhiko Kamada, Toshiro Sato, Shinta Mizuno, Makoto Naganuma, Toshiaki Teratani, Ryo Aoki, Shinji Fukuda, Wataru Suda, Masahira Hattori, Masayuki Amagai, Manabu Ohyama, Takanori Kanai

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Metabolism by the gut microbiota affects host physiology beyond the gastrointestinal tract. Here, we find that antibiotic-induced dysbiosis, in particular, overgrowth of Lactobacillus murinus (L. murinus), impaired gut metabolic function and led to the development of alopecia. While deprivation of dietary biotin per se did not affect skin physiology, its simultaneous treatment with vancomycin resulted in hair loss in specific pathogen-free (SPF) mice. Vancomycin treatment induced the accumulation of L. murinus in the gut, which consumes residual biotin and depletes available biotin in the gut. Consistently, L. murinus induced alopecia when monocolonized in germ-free mice fed a biotin-deficient diet. Supplementation of biotin can reverse established alopecia symptoms in the SPF condition, indicating that L. murinus plays a central role in the induction of hair loss via a biotin-dependent manner. Collectively, our results indicate that luminal metabolic alterations associated with gut dysbiosis and dietary modifications can compromise skin physiology.

    Original languageEnglish
    Pages (from-to)1513-1524
    Number of pages12
    JournalCell Reports
    Volume20
    Issue number7
    DOIs
    Publication statusPublished - 2017 Aug 15

    Fingerprint

    Dysbiosis
    Alopecia
    Lactobacillus
    Biotin
    Physiology
    Skin Physiological Phenomena
    Specific Pathogen-Free Organisms
    Pathogens
    Vancomycin
    Skin
    Diet Therapy
    Nutrition
    Metabolism
    Gastrointestinal Tract
    Anti-Bacterial Agents
    Diet
    Therapeutics

    Keywords

    • alopecia
    • biotin-deficiency
    • dysbiosis
    • gut microbiota
    • Lactobacillus murinus
    • metabolome
    • microbiome

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Hayashi, A., Mikami, Y., Miyamoto, K., Kamada, N., Sato, T., Mizuno, S., ... Kanai, T. (2017). Intestinal Dysbiosis and Biotin Deprivation Induce Alopecia through Overgrowth of Lactobacillus murinus in Mice. Cell Reports, 20(7), 1513-1524. https://doi.org/10.1016/j.celrep.2017.07.057

    Intestinal Dysbiosis and Biotin Deprivation Induce Alopecia through Overgrowth of Lactobacillus murinus in Mice. / Hayashi, Atsushi; Mikami, Yohei; Miyamoto, Kentaro; Kamada, Nobuhiko; Sato, Toshiro; Mizuno, Shinta; Naganuma, Makoto; Teratani, Toshiaki; Aoki, Ryo; Fukuda, Shinji; Suda, Wataru; Hattori, Masahira; Amagai, Masayuki; Ohyama, Manabu; Kanai, Takanori.

    In: Cell Reports, Vol. 20, No. 7, 15.08.2017, p. 1513-1524.

    Research output: Contribution to journalArticle

    Hayashi, A, Mikami, Y, Miyamoto, K, Kamada, N, Sato, T, Mizuno, S, Naganuma, M, Teratani, T, Aoki, R, Fukuda, S, Suda, W, Hattori, M, Amagai, M, Ohyama, M & Kanai, T 2017, 'Intestinal Dysbiosis and Biotin Deprivation Induce Alopecia through Overgrowth of Lactobacillus murinus in Mice', Cell Reports, vol. 20, no. 7, pp. 1513-1524. https://doi.org/10.1016/j.celrep.2017.07.057
    Hayashi, Atsushi ; Mikami, Yohei ; Miyamoto, Kentaro ; Kamada, Nobuhiko ; Sato, Toshiro ; Mizuno, Shinta ; Naganuma, Makoto ; Teratani, Toshiaki ; Aoki, Ryo ; Fukuda, Shinji ; Suda, Wataru ; Hattori, Masahira ; Amagai, Masayuki ; Ohyama, Manabu ; Kanai, Takanori. / Intestinal Dysbiosis and Biotin Deprivation Induce Alopecia through Overgrowth of Lactobacillus murinus in Mice. In: Cell Reports. 2017 ; Vol. 20, No. 7. pp. 1513-1524.
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