Intracellular β2-adrenergic receptor signaling specificity in mouse skeletal muscle in response to single-dose β2-agonist clenbuterol treatment and acute exercise

Shogo Sato, Ken Shirato, Ryosuke Mitsuhashi, Daisuke Inoue, Takako Kizaki, Hideki Ohno, Kaoru Tachiyashiki, Kazuhiko Imaizumi

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    The aim of this study was to clarify the intracellular β2- adrenergic receptor signaling specificity in mouse slow-twitch soleus and fast-twitch tibialis anterior (TA) muscles, resulting from single-dose β2-agonist clenbuterol treatment and acute exercise. At 1, 4, and 24 h after single-dose treatment with clenbuterol or after acute running exercise, the soleus and TA muscles were isolated and subjected to analysis. The phosphorylation of p38 mitogen-activated protein kinase (MAPK) increased after single-dose clenbuterol treatment and acute exercise in the soleus muscle but not in the TA muscle. Although there was no change in the phosphorylation of Akt after acute exercise in either muscle, phosphorylation of Akt in the soleus muscle increased after single-dose clenbuterol treatment, whereas that in the TA muscle remained unchanged. These results suggest that p38 MAPK and Akt pathways play a functional role in the adaptation to clenbuterol treatment and exercise, particularly in slow-twitch muscles.

    Original languageEnglish
    Pages (from-to)211-218
    Number of pages8
    JournalJournal of Physiological Sciences
    Volume63
    Issue number3
    DOIs
    Publication statusPublished - 2013 May

    Keywords

    • β-Adrenergic receptor signaling
    • β-Agonist clenbuterol
    • Akt
    • Exercise
    • p38 MAPK
    • Skeletal muscle

    ASJC Scopus subject areas

    • Physiology

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