Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention

Yoshiaki Takei, Atsushi Murata, Kento Yamagishi, Satoshi Arai, Hideki Nakamura, Takafumi Inoue, Shinji Takeoka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The powerful strategy of "intracellular click reaction" was used to retain a chemical Ca2+ indicator in the cytosol. Specifically, a novel clickable Ca2+ indicator "N3-fura-2 AM" was coupled with dibenzylcyclooctyl-modified biomacromolecules via copper-free click reaction in living cells and Ca2+ oscillation was observed for an extended period of time.

Original languageEnglish
Pages (from-to)7313-7315
Number of pages3
JournalChemical Communications
Volume49
Issue number66
DOIs
Publication statusPublished - 2013 Aug 25

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Fura-2
Copper
Cells

ASJC Scopus subject areas

  • Metals and Alloys
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Catalysis
  • Chemistry(all)

Cite this

Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention. / Takei, Yoshiaki; Murata, Atsushi; Yamagishi, Kento; Arai, Satoshi; Nakamura, Hideki; Inoue, Takafumi; Takeoka, Shinji.

In: Chemical Communications, Vol. 49, No. 66, 25.08.2013, p. 7313-7315.

Research output: Contribution to journalArticle

Takei, Yoshiaki ; Murata, Atsushi ; Yamagishi, Kento ; Arai, Satoshi ; Nakamura, Hideki ; Inoue, Takafumi ; Takeoka, Shinji. / Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention. In: Chemical Communications. 2013 ; Vol. 49, No. 66. pp. 7313-7315.
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