Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention

Yoshiaki Takei, Atsushi Murata, Kento Yamagishi, Satoshi Arai, Hideki Nakamura, Takafumi Inoue, Shinji Takeoka

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


The powerful strategy of “intracellular click reaction” was used to retain a chemical Ca2+ indicator in the cytosol. Specifically, a novel clickable Ca2+ indicator “N3-fura-2 AM” was coupled with dibenzylcyclooctyl-modified biomacromolecules via copper-free click reaction in living cells and Ca2+ oscillation was observed for an extended period of time.

Original languageEnglish
Pages (from-to)7313-7315
Number of pages3
JournalChemical Communications
Issue number66
Publication statusPublished - 2013 Jul 23

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry


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