Intraspecific protoplast fusion of citric acid-producing strains of Aspergillus niger

Kohtaro Kirimura, Takashi Yaguchi, Shoji Usami

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Intraspecific protoplast fusion was carried out between different citric acid-producing strains of Aspergillus niger. Protoplasts prepared from the mycelia of auxotrophic mutants were treated in 30% (w/v) polyethylene glycol solution containing 0.01 M CaCl2 and 0.5 M KCl, and fusion frequencies were 2.0-6.3%. Fusants were heterokaryons and in some cases formed sectors of the prototroph. Heterodiploids were induced from a heterokaryon by d-camphor treatment. Citric acid productivity of one heterodiploid was intermediate between those of parent strains in both shaking and solid cultures.

Original languageEnglish
Pages (from-to)473-479
Number of pages7
JournalJournal of Fermentation Technology
Volume64
Issue number6
DOIs
Publication statusPublished - 1986

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