Intraspecific protoplast fusion of citric acid-producing strains of Aspergillus niger

Kotaro Kirimura, Takashi Yaguchi, Shoji Usami

    Research output: Contribution to journalArticle

    40 Citations (Scopus)

    Abstract

    Intraspecific protoplast fusion was carried out between different citric acid-producing strains of Aspergillus niger. Protoplasts prepared from the mycelia of auxotrophic mutants were treated in 30% (w/v) polyethylene glycol solution containing 0.01 M CaCl2 and 0.5 M KCl, and fusion frequencies were 2.0-6.3%. Fusants were heterokaryons and in some cases formed sectors of the prototroph. Heterodiploids were induced from a heterokaryon by d-camphor treatment. Citric acid productivity of one heterodiploid was intermediate between those of parent strains in both shaking and solid cultures.

    Original languageEnglish
    Pages (from-to)473-479
    Number of pages7
    JournalJournal of Fermentation Technology
    Volume64
    Issue number6
    DOIs
    Publication statusPublished - 1986

    Fingerprint

    Aspergillus niger
    Protoplasts
    Aspergillus
    Citric acid
    Citric Acid
    Fusion reactions
    Camphor
    Mycelium
    Polyethylene glycols
    Productivity

    Cite this

    Intraspecific protoplast fusion of citric acid-producing strains of Aspergillus niger. / Kirimura, Kotaro; Yaguchi, Takashi; Usami, Shoji.

    In: Journal of Fermentation Technology, Vol. 64, No. 6, 1986, p. 473-479.

    Research output: Contribution to journalArticle

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