Intratumoral bidirectional transitions between epithelial and mesenchymal cells in triple-negative breast cancer

Mizuki Yamamoto, Kota Sakane, Kana Tominaga, Noriko Gotoh, Takayoshi Niwa, Yasuko Kikuchi, Keiichiro Tada, Naoki Goshima, Kentaro Senba, Jun Ichiro Inoue

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Epithelial–mesenchymal transition (EMT) and its reverse process, mesenchymal–epithelial transition MET, are crucial in several stages of cancer metastasis. Epithelial–mesenchymal transition allows cancer cells to move to proximal blood vessels for intravasation. However, because EMT and MET processes are dynamic, mesenchymal cancer cells are likely to undergo MET transiently and subsequently re-undergo EMT to restart the metastatic process. Therefore, spatiotemporally coordinated mutual regulation between EMT and MET could occur during metastasis. To elucidate such regulation, we chose HCC38, a human triple-negative breast cancer cell line, because HCC38 is composed of epithelial and mesenchymal populations at a fixed ratio even though mesenchymal cells proliferate significantly more slowly than epithelial cells. We purified epithelial and mesenchymal cells from Venus-labeled and unlabeled HCC38 cells and mixed them at various ratios to follow EMT and MET. Using this system, we found that the efficiency of EMT is approximately an order of magnitude higher than that of MET and that the two populations significantly enhance the transition of cells from the other population to their own. In addition, knockdown of Zinc finger E-box-binding homeobox 1 (ZEB1) or Zinc finger protein SNAI2 (SLUG) significantly suppressed EMT but promoted partial MET, indicating that ZEB1 and SLUG are crucial to EMT and MET. We also show that primary breast cancer cells underwent EMT that correlated with changes in expression profiles of genes determining EMT status and breast cancer subtype. These changes were very similar to those observed in EMT in HCC38 cells. Consequently, we propose HCC38 as a suitable model to analyze EMT–MET dynamics that could affect the development of triple-negative breast cancer.

    Original languageEnglish
    Pages (from-to)1210-1222
    Number of pages13
    JournalCancer Science
    Volume108
    Issue number6
    DOIs
    Publication statusPublished - 2017 Jun 1

    Fingerprint

    Triple Negative Breast Neoplasms
    Epithelial Cells
    Venus
    Breast Neoplasms
    Population
    Neoplasm Metastasis
    Neoplasms
    Zinc Fingers
    Transcriptome
    Blood Vessels
    Cell Line

    Keywords

    • Breast cancer
    • cancer development
    • EMT
    • EMT-inducing transcription factor
    • MET

    ASJC Scopus subject areas

    • Oncology
    • Cancer Research

    Cite this

    Yamamoto, M., Sakane, K., Tominaga, K., Gotoh, N., Niwa, T., Kikuchi, Y., ... Inoue, J. I. (2017). Intratumoral bidirectional transitions between epithelial and mesenchymal cells in triple-negative breast cancer. Cancer Science, 108(6), 1210-1222. https://doi.org/10.1111/cas.13246

    Intratumoral bidirectional transitions between epithelial and mesenchymal cells in triple-negative breast cancer. / Yamamoto, Mizuki; Sakane, Kota; Tominaga, Kana; Gotoh, Noriko; Niwa, Takayoshi; Kikuchi, Yasuko; Tada, Keiichiro; Goshima, Naoki; Senba, Kentaro; Inoue, Jun Ichiro.

    In: Cancer Science, Vol. 108, No. 6, 01.06.2017, p. 1210-1222.

    Research output: Contribution to journalArticle

    Yamamoto, M, Sakane, K, Tominaga, K, Gotoh, N, Niwa, T, Kikuchi, Y, Tada, K, Goshima, N, Senba, K & Inoue, JI 2017, 'Intratumoral bidirectional transitions between epithelial and mesenchymal cells in triple-negative breast cancer', Cancer Science, vol. 108, no. 6, pp. 1210-1222. https://doi.org/10.1111/cas.13246
    Yamamoto, Mizuki ; Sakane, Kota ; Tominaga, Kana ; Gotoh, Noriko ; Niwa, Takayoshi ; Kikuchi, Yasuko ; Tada, Keiichiro ; Goshima, Naoki ; Senba, Kentaro ; Inoue, Jun Ichiro. / Intratumoral bidirectional transitions between epithelial and mesenchymal cells in triple-negative breast cancer. In: Cancer Science. 2017 ; Vol. 108, No. 6. pp. 1210-1222.
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