Intravenous infusion of tridocosahexaenoyl-glycerol emulsion into rabbits effects on leukotriene B4/5 production and fatty acid composition of plasma and leukocytes

Norio Nakamura, Tomohito Hamazaki, Katsuya Yamazaki, Hirofumi Taki, Masashi Kobayashi, Kazunaga Yazawa, Fumio Ibuki

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22 Citations (Scopus)

Abstract

Leukotriene (LT) B4 is a major chemical activator of PMN. Inhibitory effects of oral administration of docosahexaenoic acid (DHA) on LTB4 synthesis by PMN are known. We intravenously infused tridocosahexaenoyl-glycerol (DHA-TG) emulsion into rabbits in three different doses, namely 0.8, 0.4, or 0.2 g DHA/kg, and investigated the changes in LTB4/5 production by ionophore-activated PMN. The averaged LTB4 production by PMN was significantly reduced to 57 and 59% of baseline at 6 h after the infusion of 0.8 and 0.4 g DHA/kg, respectively (P < 0.05), but not after the infusion of 0.2 g DHA/kg or 0.8 g soybean oil/kg. The combined concentrations of both DHA and eicosapentaenoic acid in the PMN phospholipid fraction were significantly increased at 6 h after the infusion of 0.8 or 0.4 g DHA/kg but not after the infusion of 0.2 g DHA/kg or 0.8 g soybean oil/kg. Oral administration of 0.8 g DHA/kg did not increase DHA or eicosapentaenoic acid in the PMN phospholipid fraction and did not decrease LTB4 production by PMN at 6 h after administration. We suggest that the infusion of 0.4-0.8 g DHA/kg might be beneficial to patients who suffer from diseases that are related to the acute elevation of LTB4 production.

Original languageEnglish
Pages (from-to)1253-1261
Number of pages9
JournalJournal of Clinical Investigation
Volume92
Issue number3
Publication statusPublished - 1993 Sep
Externally publishedYes

Keywords

  • Arachidonic acid
  • Docosahexaenoic acid
  • Dose dependency
  • Inflammation
  • Phospholipids

ASJC Scopus subject areas

  • Medicine(all)

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