Intron Length Variation Observed in the Creatine Kinase and Ribosomal Protein Genes of the Swordfish Xiphias gladius

Seinen Chow, Haruko Takeyama

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Introns may accumulate much higher genetic variation than exons. Universal primers were designed from the conservative nucleotide sequences of exons to amplify the flanking intron. Length variations in the S7 ribosomal protein (RP) gene intron 1 and mitochondrial creatine kinase (CK) gene intron 6 of the swordfish Xiphias gladius were found. Single or two banded fragment patterns in each individual were observed by agarose gel electrophoresis. Nucleotide sequence analysis revealed that highly polymorphic fragment patterns observed in the RP gene intron 1 were due to different numbers of a TG repeat (microsatellite). The length of the CK gene intron 6 was dimorphic, in which presence or absence of a 24 bp block was responsible for longer or shorter introns. Additional minor nucleotide insertion/deletions were observed independent of the RP microsatellite and the CK 24 bp block regions. The results of this investigation indicate that introns may be good sources of intraspecific genetic variation for population genetic studies and that the same set of primers can be used to amplify homologous intron regions even among distant species. Further, the conserved exon primed PCR strategy may be useful to prevent appearance of priming site polymorphism (null allele).

Original languageEnglish
Pages (from-to)397-402
Number of pages6
JournalFisheries Science
Volume64
Issue number3
Publication statusPublished - 1998 Jun
Externally publishedYes

Fingerprint

Xiphias gladius
ribosomal proteins
creatine kinase
introns
protein
gene
genetic variation
genes
exons
population genetics
electrokinesis
allele
polymorphism
gel
microsatellite repeats
nucleotide sequences
null alleles
agarose
gel electrophoresis
sequence analysis

Keywords

  • Fish population genetics
  • Intron length polymorphism
  • Swordfish

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Intron Length Variation Observed in the Creatine Kinase and Ribosomal Protein Genes of the Swordfish Xiphias gladius. / Chow, Seinen; Takeyama, Haruko.

In: Fisheries Science, Vol. 64, No. 3, 06.1998, p. 397-402.

Research output: Contribution to journalArticle

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