Abstract
The study of intracellular water in living cells is a challenge of fundamental importance. While the critical roles of water in maintaining and propagating life have been widely recognized and accepted, our understanding of water/biomolecule interactions is surprisingly limited. Using Raman microspectroscopic imaging of a living yeast cell followed by a multivariate analysis in form of the nonnegative matrix factorization method, we successfully resolve organelle-specific water structures. The intensities and the band profiles of the segregated water OH stretch spectra yield important and otherwise unobtainable information on the extensive effect of the water/biomolecule interactions in a given organelle on the hydrogen-bonding network of water molecules.
| Original language | English |
|---|---|
| Pages (from-to) | 167-169 |
| Number of pages | 3 |
| Journal | Journal of Raman Spectroscopy |
| Volume | 44 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2013 Feb |
| Externally published | Yes |
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Keywords
- imaging
- living cells
- multivariate analysis
- Raman microspectroscopy
- water
ASJC Scopus subject areas
- Spectroscopy
- Materials Science(all)
Cite this
Investigation of organelle-specific intracellular water structures with Raman microspectroscopy. / Tiwari, Shraeddha; Ando, Masahiro; Hamaguchi, Hiro O.
In: Journal of Raman Spectroscopy, Vol. 44, No. 2, 02.2013, p. 167-169.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Investigation of organelle-specific intracellular water structures with Raman microspectroscopy
AU - Tiwari, Shraeddha
AU - Ando, Masahiro
AU - Hamaguchi, Hiro O.
PY - 2013/2
Y1 - 2013/2
N2 - The study of intracellular water in living cells is a challenge of fundamental importance. While the critical roles of water in maintaining and propagating life have been widely recognized and accepted, our understanding of water/biomolecule interactions is surprisingly limited. Using Raman microspectroscopic imaging of a living yeast cell followed by a multivariate analysis in form of the nonnegative matrix factorization method, we successfully resolve organelle-specific water structures. The intensities and the band profiles of the segregated water OH stretch spectra yield important and otherwise unobtainable information on the extensive effect of the water/biomolecule interactions in a given organelle on the hydrogen-bonding network of water molecules.
AB - The study of intracellular water in living cells is a challenge of fundamental importance. While the critical roles of water in maintaining and propagating life have been widely recognized and accepted, our understanding of water/biomolecule interactions is surprisingly limited. Using Raman microspectroscopic imaging of a living yeast cell followed by a multivariate analysis in form of the nonnegative matrix factorization method, we successfully resolve organelle-specific water structures. The intensities and the band profiles of the segregated water OH stretch spectra yield important and otherwise unobtainable information on the extensive effect of the water/biomolecule interactions in a given organelle on the hydrogen-bonding network of water molecules.
KW - imaging
KW - living cells
KW - multivariate analysis
KW - Raman microspectroscopy
KW - water
UR - http://www.scopus.com/inward/record.url?scp=84872978356&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84872978356&partnerID=8YFLogxK
U2 - 10.1002/jrs.4165
DO - 10.1002/jrs.4165
M3 - Article
VL - 44
SP - 167
EP - 169
JO - Journal of Raman Spectroscopy
JF - Journal of Raman Spectroscopy
SN - 0377-0486
IS - 2
ER -