Investigation of organelle-specific intracellular water structures with Raman microspectroscopy

Shraeddha Tiwari, Masahiro Ando, Hiro O. Hamaguchi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The study of intracellular water in living cells is a challenge of fundamental importance. While the critical roles of water in maintaining and propagating life have been widely recognized and accepted, our understanding of water/biomolecule interactions is surprisingly limited. Using Raman microspectroscopic imaging of a living yeast cell followed by a multivariate analysis in form of the nonnegative matrix factorization method, we successfully resolve organelle-specific water structures. The intensities and the band profiles of the segregated water OH stretch spectra yield important and otherwise unobtainable information on the extensive effect of the water/biomolecule interactions in a given organelle on the hydrogen-bonding network of water molecules.

Original languageEnglish
Pages (from-to)167-169
Number of pages3
JournalJournal of Raman Spectroscopy
Volume44
Issue number2
DOIs
Publication statusPublished - 2013 Feb
Externally publishedYes

Fingerprint

Water
Biomolecules
Cells
Factorization
Yeast
Hydrogen bonds
Imaging techniques
Molecules

Keywords

  • imaging
  • living cells
  • multivariate analysis
  • Raman microspectroscopy
  • water

ASJC Scopus subject areas

  • Spectroscopy
  • Materials Science(all)

Cite this

Investigation of organelle-specific intracellular water structures with Raman microspectroscopy. / Tiwari, Shraeddha; Ando, Masahiro; Hamaguchi, Hiro O.

In: Journal of Raman Spectroscopy, Vol. 44, No. 2, 02.2013, p. 167-169.

Research output: Contribution to journalArticle

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