Investigation of organelle-specific intracellular water structures with Raman microspectroscopy

Shraeddha Tiwari, Masahiro Ando, Hiro O. Hamaguchi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The study of intracellular water in living cells is a challenge of fundamental importance. While the critical roles of water in maintaining and propagating life have been widely recognized and accepted, our understanding of water/biomolecule interactions is surprisingly limited. Using Raman microspectroscopic imaging of a living yeast cell followed by a multivariate analysis in form of the nonnegative matrix factorization method, we successfully resolve organelle-specific water structures. The intensities and the band profiles of the segregated water OH stretch spectra yield important and otherwise unobtainable information on the extensive effect of the water/biomolecule interactions in a given organelle on the hydrogen-bonding network of water molecules.

Original languageEnglish
Pages (from-to)167-169
Number of pages3
JournalJournal of Raman Spectroscopy
Volume44
Issue number2
DOIs
Publication statusPublished - 2013 Feb 1
Externally publishedYes

Keywords

  • Raman microspectroscopy
  • imaging
  • living cells
  • multivariate analysis
  • water

ASJC Scopus subject areas

  • Materials Science(all)
  • Spectroscopy

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