Abstract
The study of intracellular water in living cells is a challenge of fundamental importance. While the critical roles of water in maintaining and propagating life have been widely recognized and accepted, our understanding of water/biomolecule interactions is surprisingly limited. Using Raman microspectroscopic imaging of a living yeast cell followed by a multivariate analysis in form of the nonnegative matrix factorization method, we successfully resolve organelle-specific water structures. The intensities and the band profiles of the segregated water OH stretch spectra yield important and otherwise unobtainable information on the extensive effect of the water/biomolecule interactions in a given organelle on the hydrogen-bonding network of water molecules.
Original language | English |
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Pages (from-to) | 167-169 |
Number of pages | 3 |
Journal | Journal of Raman Spectroscopy |
Volume | 44 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2013 Feb 1 |
Externally published | Yes |
Keywords
- Raman microspectroscopy
- imaging
- living cells
- multivariate analysis
- water
ASJC Scopus subject areas
- Materials Science(all)
- Spectroscopy