Investigation of the Antibacterial Activity and in vivo Cytotoxicity of Biogenic Silver Nanoparticles as Potent Therapeutics

Md Monir Hossain, Shakil Ahmed Polash, Masato Takikawa, Razib Datta Shubhra, Tanushree Saha, Zinia Islam, Sharif Hossain, Md Ashraful Hasan, Shinji Takeoka, Satya Ranjan Sarker

Research output: Contribution to journalArticle

Abstract

Biogenic nanoparticles are the smartest weapons to deal with the multidrug-resistant “superbugs” because of their broad-spectrum antibacterial propensity as well as excellent biocompatibility. The aqueous biogenic silver nanoparticles (Aq-bAgNPs) and ethanolic biogenic silver nanoparticles (Et-bAgNPs) were synthesized using aqueous and ethanolic extracts of Andrographis paniculata stem, respectively, as reducing agents. Electron microscopic images confirmed the synthesis of almost spherical shaped biogenic silver nanoparticles (bAgNPs). The zeta potentials of the nanoparticles were negative and were −22 and −26 mV for Aq-bAgNPs and Et-bAgNPs, respectively. The antibacterial activity of bAgNPs was investigated against seven pathogenic (i.e., enteropathogenic Escherichia coli, Salmonella typhi, Staphylococcus aureus, Vibrio cholerae, Enterococcus faecalis, Hafnia alvei, Acinetobacter baumannii) and three nonpathogenic (i.e., E. coli DH5α, E. coli K12, and Bacillus subtilis) bacteria at different time points (i.e., 12, 16, 20, and 24 h) in a dose-dependent manner (i.e., 20, 40, and 60 μg) through broth dilution assay, disk diffusion assay, CellToxTM Green uptake assay, and trypan blue dye exclusion assay. The lowest minimum inhibitory concentration value for both the bAgNPs was 0.125 μg. Et-bAgNPs showed the highest antibacterial activity against S. aureus at 60 μg after 16 h and the diameter of inhibited zone was 28 mm. Lipid peroxidation assay using all the bacterial strains revealed the formation of malondialdehyde–thiobarbituric acid adduct due to the oxidation of cell membrane fatty acids by bAgNPs. The bAgNPs showed excellent hemocompatibility against human as well as rat red blood cells. Furthermore, there was no significant toxicity observed when the levels of rat serum ALT, AST, γ-GT (i.e., liver function biomarkers), and creatinine (i.e., kidney function biomarker) were determined.

Original languageEnglish
Article number239
JournalFrontiers in Bioengineering and Biotechnology
Volume7
DOIs
Publication statusPublished - 2019 Oct 9

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Cytotoxicity
Silver
Nanoparticles
Assays
Escherichia coli
Biomarkers
Staphylococcus aureus
Rats
Hafnia alvei
Andrographis
Therapeutics
Enteropathogenic Escherichia coli
Escherichia coli K12
Acinetobacter baumannii
Salmonella typhi
Salmonella
Weapons
Vibrio cholerae
Trypan Blue
Enterococcus faecalis

Keywords

  • antimicrobial activity
  • biocompatibility
  • biogenic silver nanoparticles
  • biomarkers
  • CellTox green assay
  • hemocompatibility
  • lipid peroxidation
  • pathogenic bacteria

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Histology
  • Biomedical Engineering

Cite this

Investigation of the Antibacterial Activity and in vivo Cytotoxicity of Biogenic Silver Nanoparticles as Potent Therapeutics. / Hossain, Md Monir; Polash, Shakil Ahmed; Takikawa, Masato; Shubhra, Razib Datta; Saha, Tanushree; Islam, Zinia; Hossain, Sharif; Hasan, Md Ashraful; Takeoka, Shinji; Sarker, Satya Ranjan.

In: Frontiers in Bioengineering and Biotechnology, Vol. 7, 239, 09.10.2019.

Research output: Contribution to journalArticle

Hossain, Md Monir ; Polash, Shakil Ahmed ; Takikawa, Masato ; Shubhra, Razib Datta ; Saha, Tanushree ; Islam, Zinia ; Hossain, Sharif ; Hasan, Md Ashraful ; Takeoka, Shinji ; Sarker, Satya Ranjan. / Investigation of the Antibacterial Activity and in vivo Cytotoxicity of Biogenic Silver Nanoparticles as Potent Therapeutics. In: Frontiers in Bioengineering and Biotechnology. 2019 ; Vol. 7.
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AU - Saha, Tanushree

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AU - Takeoka, Shinji

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