Investigation of the antiviral properties of copper iodide nanoparticles against feline calicivirus

Nozomi Shionoiri, Tetsuya Sato, Yoshie Fujimori, Tsuruo Nakayama, Michiko Nemoto, Tadashi Matsunaga, Tsuyoshi Tanaka

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

This study demonstrated the antiviral properties of copper iodide (CuI) nanoparticles against the non-enveloped virus feline calicivirus (FCV) as a surrogate for human norovirus. The effect of CuI nanoparticles on FCV infectivity to Crandell-Rees feline kidney (CRFK) cells was elucidated. The infectivity of FCV to CRFK cells was greatly reduced by 7 orders of magnitude at 1000μgml-1 CuI nanoparticles. At the conditions, electron spin resonance (ESR) analysis proved hydroxyl radical production in CuI nanoparticle suspension. Furthermore, amino acid oxidation in the viral capsid protein of FCV was determined by nanoflow liquid chromatography-mass spectrometric (nano LC-MS) analysis. The use of CuI nanoparticles showed extremely high antiviral activity against FCV. The high antiviral property of CuI nanoparticles was attributed to Cu+, followed by ROS generation and subsequent capsid protein oxidation. CuI nanoparticles could be proposed as useful sources of a continuous supply of Cu+ ions for efficient virus inactivation. Furthermore, this study brings new insights into toxic actions of copper iodide nanoparticles against viruses.

Original languageEnglish
Pages (from-to)580-586
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume113
Issue number5
DOIs
Publication statusPublished - 2012 May
Externally publishedYes

Fingerprint

Feline Calicivirus
Iodides
Nanoparticles
Antiviral Agents
Copper
Viruses
Felidae
Capsid Proteins
Virus Inactivation
Proteins
Kidney
Toxic Actions
Norovirus
Oxidation
Liquid chromatography
Electron Spin Resonance Spectroscopy
Viral Proteins
Liquid Chromatography
Hydroxyl Radical
Paramagnetic resonance

Keywords

  • Amino acid oxidation
  • Antiviral activity
  • Copper iodide (CuI)
  • Feline calicivirus (FCV)
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Investigation of the antiviral properties of copper iodide nanoparticles against feline calicivirus. / Shionoiri, Nozomi; Sato, Tetsuya; Fujimori, Yoshie; Nakayama, Tsuruo; Nemoto, Michiko; Matsunaga, Tadashi; Tanaka, Tsuyoshi.

In: Journal of Bioscience and Bioengineering, Vol. 113, No. 5, 05.2012, p. 580-586.

Research output: Contribution to journalArticle

Shionoiri, Nozomi ; Sato, Tetsuya ; Fujimori, Yoshie ; Nakayama, Tsuruo ; Nemoto, Michiko ; Matsunaga, Tadashi ; Tanaka, Tsuyoshi. / Investigation of the antiviral properties of copper iodide nanoparticles against feline calicivirus. In: Journal of Bioscience and Bioengineering. 2012 ; Vol. 113, No. 5. pp. 580-586.
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