Involvement of androgen receptor in sex determination in an amphibian species

Jun Fujii, Maho Kodama, Akira Oike, Yasuki Matsuo, Mi Sook Min, Takashi Hasebe, Atsuko Ishizuya-Oka, Koichi Kawakami, Masahisa Nakamura

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    In mice and humans, the androgen receptor (AR) gene, located on the X chromosome, is not known to be involved in sex determination. In the Japanese frog Rana rugosa the AR is located on the sex chromosomes (X, Y, Z and W). Phylogenetic analysis shows that the AR on the X chromosome (X-AR) of the Korean R. rugosa is basal and segregates into two clusters: one containing W-AR of Japanese R. rugosa, the other containing Y-AR. AR expression is twice as high in ZZ (male) compared to ZW (female) embryos in which the W-AR is barely expressed. Higher AR-expression may be associated with male sex determination in this species. To examine whether the Z-AR is involved in sex determination in R. rugosa, we produced transgenic (Tg) frogs carrying an exogenous Z-AR. Analysis of ZW Tg frogs revealed development of masculinized gonads or 'ovotestes'. Expression of CYP17 and Dmrt1, genes known to be activated during normal male gonadal development, were up-regulated in the ZW ovotestis. Testosterone, supplied to the rearing water, completed the female-to-male sex-reversal in the AR-Tg ZW frogs. Here we report that Z-AR is involved in male sex-determination in an amphibian species.

    Original languageEnglish
    Article numbere93655
    JournalPLoS One
    Volume9
    Issue number5
    DOIs
    Publication statusPublished - 2014 May 14

    Fingerprint

    Androgen Receptors
    Amphibians
    amphibians
    gender
    Anura
    frogs
    X Chromosome
    Chromosomes
    genetically modified organisms
    X chromosome
    androgen receptors
    Genes
    Steroid 17-alpha-Hydroxylase
    Ranidae
    Sex Chromosomes
    Gonads
    sex reversal
    sex chromosomes
    Testosterone
    testosterone

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Fujii, J., Kodama, M., Oike, A., Matsuo, Y., Min, M. S., Hasebe, T., ... Nakamura, M. (2014). Involvement of androgen receptor in sex determination in an amphibian species. PLoS One, 9(5), [e93655]. https://doi.org/10.1371/journal.pone.0093655

    Involvement of androgen receptor in sex determination in an amphibian species. / Fujii, Jun; Kodama, Maho; Oike, Akira; Matsuo, Yasuki; Min, Mi Sook; Hasebe, Takashi; Ishizuya-Oka, Atsuko; Kawakami, Koichi; Nakamura, Masahisa.

    In: PLoS One, Vol. 9, No. 5, e93655, 14.05.2014.

    Research output: Contribution to journalArticle

    Fujii, J, Kodama, M, Oike, A, Matsuo, Y, Min, MS, Hasebe, T, Ishizuya-Oka, A, Kawakami, K & Nakamura, M 2014, 'Involvement of androgen receptor in sex determination in an amphibian species', PLoS One, vol. 9, no. 5, e93655. https://doi.org/10.1371/journal.pone.0093655
    Fujii J, Kodama M, Oike A, Matsuo Y, Min MS, Hasebe T et al. Involvement of androgen receptor in sex determination in an amphibian species. PLoS One. 2014 May 14;9(5). e93655. https://doi.org/10.1371/journal.pone.0093655
    Fujii, Jun ; Kodama, Maho ; Oike, Akira ; Matsuo, Yasuki ; Min, Mi Sook ; Hasebe, Takashi ; Ishizuya-Oka, Atsuko ; Kawakami, Koichi ; Nakamura, Masahisa. / Involvement of androgen receptor in sex determination in an amphibian species. In: PLoS One. 2014 ; Vol. 9, No. 5.
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