Involvement of androgen receptor in sex determination in an amphibian species

Jun Fujii, Maho Kodama, Akira Oike, Yasuki Matsuo, Mi Sook Min, Takashi Hasebe, Atsuko Ishizuya-Oka, Koichi Kawakami, Masahisa Nakamura

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    In mice and humans, the androgen receptor (AR) gene, located on the X chromosome, is not known to be involved in sex determination. In the Japanese frog Rana rugosa the AR is located on the sex chromosomes (X, Y, Z and W). Phylogenetic analysis shows that the AR on the X chromosome (X-AR) of the Korean R. rugosa is basal and segregates into two clusters: one containing W-AR of Japanese R. rugosa, the other containing Y-AR. AR expression is twice as high in ZZ (male) compared to ZW (female) embryos in which the W-AR is barely expressed. Higher AR-expression may be associated with male sex determination in this species. To examine whether the Z-AR is involved in sex determination in R. rugosa, we produced transgenic (Tg) frogs carrying an exogenous Z-AR. Analysis of ZW Tg frogs revealed development of masculinized gonads or 'ovotestes'. Expression of CYP17 and Dmrt1, genes known to be activated during normal male gonadal development, were up-regulated in the ZW ovotestis. Testosterone, supplied to the rearing water, completed the female-to-male sex-reversal in the AR-Tg ZW frogs. Here we report that Z-AR is involved in male sex-determination in an amphibian species.

    Original languageEnglish
    Article numbere93655
    JournalPLoS One
    Volume9
    Issue number5
    DOIs
    Publication statusPublished - 2014 May 14

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

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  • Cite this

    Fujii, J., Kodama, M., Oike, A., Matsuo, Y., Min, M. S., Hasebe, T., Ishizuya-Oka, A., Kawakami, K., & Nakamura, M. (2014). Involvement of androgen receptor in sex determination in an amphibian species. PLoS One, 9(5), [e93655]. https://doi.org/10.1371/journal.pone.0093655