Involvement of Gαs-proteins in the action of relaxin-like gonad-stimulating substance on starfish ovarian follicle cells

Masatoshi Mita, Shogo Haraguchi, Miho Watanabe, Yuki Takeshige, Kazutoshi Yamamoto, Kazuyoshi Tsutsui

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Gonad-stimulating substance (GSS) in starfish is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to gonadotropins in vertebrates. In breeding season (stage V), GSS stimulates oocyte maturation to induce 1-methyladenine (1-MeAde) by ovarian follicle cells. The hormonal action of GSS is mediated through the activation of its receptor, G-proteins and adenylyl cyclase. It has been reported that GSS fails to induce 1-MeAde and cyclic AMP (cAMP) production in follicle cells of ovaries during oogenesis (stage IV). This study examined the regulatory mechanism how ovarian follicle cells acquire the potential to respond to GSS by producing 1-MeAde and cAMP. Because the failure of GSS action was due to G-proteins of follicle cells, the molecular structures of Gα. s, Gα. i, Gα. q and Gβ were identified in follicle cells of starfish Asterina pectinifera. The cDNA sequences of Gα. s, Gα. i, Gα. q and Gβ consisted of ORFs encoding 379, 354, 353 and 353 amino acids. The expression levels of Gα. s were extremely low in follicle cells at stage IV, whereas the mRNA levels increased markedly in stage V. On contrary, the mRNA levels of Gα. i were almost constant regardless of stage IV and V. These findings strongly suggest that de novo synthesis of Gα. s-proteins is contributed to the action of GSS on follicle cells to produce 1-MeAde and cAMP.

    Original languageEnglish
    Pages (from-to)80-87
    Number of pages8
    JournalGeneral and Comparative Endocrinology
    Volume205
    DOIs
    Publication statusPublished - 2014 Sep 1

    Fingerprint

    relaxin
    Starfish
    Relaxin
    Ovarian Follicle
    Asteroidea
    ovarian follicles
    Gonads
    gonads
    cyclic AMP
    Proteins
    Cyclic AMP
    proteins
    cells
    G-proteins
    GTP-Binding Proteins
    Invertebrate Hormones
    Asterina
    Asterina pectinifera
    peptide hormones
    Oogenesis

    Keywords

    • G-proteins
    • Gonad-stimulating substance (GSS)
    • MRNA expression
    • Ovarian follicle cells
    • Starfish

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Involvement of Gαs-proteins in the action of relaxin-like gonad-stimulating substance on starfish ovarian follicle cells. / Mita, Masatoshi; Haraguchi, Shogo; Watanabe, Miho; Takeshige, Yuki; Yamamoto, Kazutoshi; Tsutsui, Kazuyoshi.

    In: General and Comparative Endocrinology, Vol. 205, 01.09.2014, p. 80-87.

    Research output: Contribution to journalArticle

    Mita, Masatoshi ; Haraguchi, Shogo ; Watanabe, Miho ; Takeshige, Yuki ; Yamamoto, Kazutoshi ; Tsutsui, Kazuyoshi. / Involvement of Gαs-proteins in the action of relaxin-like gonad-stimulating substance on starfish ovarian follicle cells. In: General and Comparative Endocrinology. 2014 ; Vol. 205. pp. 80-87.
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