Involvement of glutamate release in substance P-induced phase delays of suprachiasmatic neuron activity rhythm in vitro

Toshiyuki Hamada, Shiro Yamanouchi, Akihito Watanabe, Shigenobu Shibata, Shigenori Watanabe

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24 Citations (Scopus)


The suprachiasmatic nucleus (SCN) has been identified as a mammalian circadian rhythm clock. Treatment with substance P (SP) at zeitgeber time 13- 14 produced phase delays of circadian rhythm in spontaneous neural activity in SCN neurons in vitro. SP-induced phase delays are blocked by treatment with not only SP receptor antagonist, spantide, but N-methyl-D-aspartate receptor antagonist, MK-801. In the biochemical experiment, we demonstrated that SP-induced glutamate release from the SCN slices was observed by the high-performance liquid chromatography assay. The present results suggest that glutamate release may be involved in SP-induced phase delays.

Original languageEnglish
Pages (from-to)190-193
Number of pages4
JournalBrain Research
Issue number1-2
Publication statusPublished - 1999 Jul 31



  • Circadian rhythm
  • Firing rhythm
  • Glutamate release
  • NMDA receptor antagonist
  • Substance P
  • Suprachiasmatic nucleus

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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