Involvement of neutrophil dynamics and function in exercise-induced muscle damage and delayed-onset muscle soreness: Effect of hydrogen bath

Takuji Kawamura, Katsuhiko Suzuki, Masaki Takahashi, Miki Tomari, Reira Hara, Yuko Gando, Isao Muraoka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The purpose of this study was to investigate the involvement of neutrophil dynamics and function in exercise-induced muscle damage (EIMD) and delayed-onset muscle soreness (DOMS), and the effect of molecular hydrogen (H2 ) intake on these parameters. Nine healthy and active young men performed H2 and placebo bath trial in a crossover design. They carried out downhill running (−8% slope) for 30 min at a speed corresponding to 75~85% of peak oxygen uptake (VO2 peak). Subsequently, they repeated bathing for 20 min per day for one week. Degree of muscle soreness (visual analogue scale: VAS), peripheral leukocyte counts, neutrophil dynamics and function, muscle damage, and inflammation markers were measured. Plasma interleukin (IL)-6 concentration was significantly correlated with peripheral neutrophil count, VAS, and serum creatine kinase activity, respectively, after downhill running. Peripheral neutrophil count and serum myoglobin concentration were also significantly correlated. Conversely, there were no effects of H2 bath. These results suggest that IL-6 may be involved in the mobilization of neutrophils into the peripheral blood and subsequent EIMD and DOMS after downhill running; however, it is not likely that H2 bath is effective for the inflammatory process that was centered on neutrophils after downhill running.

Original languageEnglish
Article number, 127;
JournalAntioxidants
Volume7
Issue number10
DOIs
Publication statusPublished - 2018 Oct 1

Fingerprint

Myalgia
Baths
Muscle
Hydrogen
Neutrophils
Exercise
Running
Muscles
Interleukin-6
Myoglobin
Creatine Kinase
Serum
Visual Analog Scale
Leukocyte Count
Cross-Over Studies
Blood
Placebos
Oxygen
Inflammation
Plasmas

Keywords

  • Delayed-onset muscle soreness
  • Downhill running
  • Inflammation
  • Molecular hydrogen
  • Muscle damage
  • Neutrophil
  • Oxidative stress

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

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abstract = "The purpose of this study was to investigate the involvement of neutrophil dynamics and function in exercise-induced muscle damage (EIMD) and delayed-onset muscle soreness (DOMS), and the effect of molecular hydrogen (H2 ) intake on these parameters. Nine healthy and active young men performed H2 and placebo bath trial in a crossover design. They carried out downhill running (−8{\%} slope) for 30 min at a speed corresponding to 75~85{\%} of peak oxygen uptake (VO2 peak). Subsequently, they repeated bathing for 20 min per day for one week. Degree of muscle soreness (visual analogue scale: VAS), peripheral leukocyte counts, neutrophil dynamics and function, muscle damage, and inflammation markers were measured. Plasma interleukin (IL)-6 concentration was significantly correlated with peripheral neutrophil count, VAS, and serum creatine kinase activity, respectively, after downhill running. Peripheral neutrophil count and serum myoglobin concentration were also significantly correlated. Conversely, there were no effects of H2 bath. These results suggest that IL-6 may be involved in the mobilization of neutrophils into the peripheral blood and subsequent EIMD and DOMS after downhill running; however, it is not likely that H2 bath is effective for the inflammatory process that was centered on neutrophils after downhill running.",
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AU - Suzuki, Katsuhiko

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AU - Hara, Reira

AU - Gando, Yuko

AU - Muraoka, Isao

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KW - Muscle damage

KW - Neutrophil

KW - Oxidative stress

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