Involvement of sulfoquinovosyl diacylglycerol in the structural integrity and heat-tolerance of photosystem II

Norihiro Sato, Motohide Aoki, Yukihiro Maru, Kintake Sonoike, Ayumi Minoda, Mikio Tsuzuki

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

To examine the role of sulfoquinovosyl diacylglycerol (SQDG) in thylakoid membranes, we compared the structural and functional properties of photosystem II (PSII) between a mutant of Chlamydomonas reinhardtii defective in SQDG (hf-2) and the wild type. The PSII core complex of hf-2, as compared with that of the wild type, showed structural fragility when solubilized with a detergent, dodecyl β-D-maltoside, suggesting that the physical properties of the PSII complex were altered by the loss of SQDG. On the other hand, exposure of the cells to 41°C for 120 min in the dark decreased the PSII activity to 70% and 50% of the initial levels in the wild type and hf-2, respectively, which implies that the PSII activity, in the absence of SQDG, becomes less stable under heat-stress conditions. PSII inactivated to 60% of the initial level by dark incubation at 41°C was reactivated by following illumination even at 41°C to more than 90% in the wild type, but only to 70% in hf-2. These results suggest that PSII inactivated by heat recovers through some mechanism dependent on light, and that SQDG participates in functioning of the mechanism. The conformational disorder of PSII caused by the defect in SQDG might be correlated with the increased susceptibility of its activity to heat-stress.

Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalPlanta
Volume217
Issue number2
Publication statusPublished - 2003 Jun 1
Externally publishedYes

Fingerprint

Photosystem II Protein Complex
Diglycerides
diacylglycerols
heat tolerance
photosystem II
Hot Temperature
heat stress
Chlamydomonas reinhardtii
Thylakoids
Thermotolerance
Lighting
thylakoids
detergents
Detergents
functional properties
lighting
physical properties
heat
Light
mutants

Keywords

  • Chlamydomonas
  • Dodecyl β-D-maltoside
  • Heat stress
  • Herbicide
  • Photosystem II
  • Sulfoquinovosyl diacylglycerol

ASJC Scopus subject areas

  • Plant Science

Cite this

Involvement of sulfoquinovosyl diacylglycerol in the structural integrity and heat-tolerance of photosystem II. / Sato, Norihiro; Aoki, Motohide; Maru, Yukihiro; Sonoike, Kintake; Minoda, Ayumi; Tsuzuki, Mikio.

In: Planta, Vol. 217, No. 2, 01.06.2003, p. 245-251.

Research output: Contribution to journalArticle

Sato, N, Aoki, M, Maru, Y, Sonoike, K, Minoda, A & Tsuzuki, M 2003, 'Involvement of sulfoquinovosyl diacylglycerol in the structural integrity and heat-tolerance of photosystem II', Planta, vol. 217, no. 2, pp. 245-251.
Sato, Norihiro ; Aoki, Motohide ; Maru, Yukihiro ; Sonoike, Kintake ; Minoda, Ayumi ; Tsuzuki, Mikio. / Involvement of sulfoquinovosyl diacylglycerol in the structural integrity and heat-tolerance of photosystem II. In: Planta. 2003 ; Vol. 217, No. 2. pp. 245-251.
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