Involvement of the neurosteroid 7α-hydroxypregnenolone in the courtship behavior of the male newt Cynops pyrrhogaster

Fumiyo Toyoda, Itaru Hasunuma, Tomoaki Nakada, Shogo Haraguchi, Kazuyoshi Tsutsui, Sakae Kikuyama

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Reproductive behavior in amphibians, as in other vertebrate animals, is controlled by multiple hormones. A neurosteroid, 7α-hydroxypregnenolone, has recently been found to enhance locomotor activity in the male newt, Cynops pyrrhogaster. Here, we show that this neurosteroid is also involved in enhancing the expression of courtship behavior. Intracerebroventricular (ICV) injection of 7α-hydroxypregnenolone enhanced courtship behavior dose-dependently in the sexually undeveloped males that had been pretreated with prolactin and gonadotropin, which is known to bring the males to a sexually developed state. But, unlike the case in the locomotion activity, 7α-hydroxypregnenolone did not elicit the behavior in males receiving no prior injections of these hormones. ICV administration of ketoconazole, an inhibitor of the steroidogenic enzyme cytochrome P450s, suppressed the spontaneously occurring courtship behavior in sexually active males. Supplementation with 7α-hydroxypregnenolone reversed the effect of ketoconazole in these animals. It was also demonstrated that the effect of the neurosteroid on the courtship behavior was blocked by a dopamine D2-like, but not by a D1-like, receptor antagonist. These results indicate that endogenous 7α-hydroxypregnenolone enhances the expression of the male courtship behavior through a dopaminergic system mediated by a D2-like receptor in the brain.

    Original languageEnglish
    Pages (from-to)375-380
    Number of pages6
    JournalHormones and Behavior
    Volume62
    Issue number4
    DOIs
    Publication statusPublished - 2012 Sep

    Fingerprint

    Salamandridae
    Courtship
    Neurotransmitter Agents
    Ketoconazole
    Locomotion
    Hormones
    Reproductive Behavior
    Injections
    Amphibians
    Enzyme Inhibitors
    Cytochromes
    Gonadotropins
    Prolactin
    Vertebrates
    7-hydroxypregnenolone
    Dopamine
    Brain

    Keywords

    • 7α-hydroxypregnenolone
    • Behavior
    • Courtship
    • Dopamine
    • Neurosteroid
    • Newt

    ASJC Scopus subject areas

    • Endocrinology
    • Behavioral Neuroscience
    • Endocrine and Autonomic Systems

    Cite this

    Involvement of the neurosteroid 7α-hydroxypregnenolone in the courtship behavior of the male newt Cynops pyrrhogaster. / Toyoda, Fumiyo; Hasunuma, Itaru; Nakada, Tomoaki; Haraguchi, Shogo; Tsutsui, Kazuyoshi; Kikuyama, Sakae.

    In: Hormones and Behavior, Vol. 62, No. 4, 09.2012, p. 375-380.

    Research output: Contribution to journalArticle

    Toyoda, Fumiyo ; Hasunuma, Itaru ; Nakada, Tomoaki ; Haraguchi, Shogo ; Tsutsui, Kazuyoshi ; Kikuyama, Sakae. / Involvement of the neurosteroid 7α-hydroxypregnenolone in the courtship behavior of the male newt Cynops pyrrhogaster. In: Hormones and Behavior. 2012 ; Vol. 62, No. 4. pp. 375-380.
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